RP-HPLC测定人血浆中盐酸二甲双胍的浓度  

Determination of Metformin in Human Plasma by RP-HPLC

作　　者：曾晓晖[1] 石磊[1] 罗新根[1] 赵玮[1] 张强[1] 余昆松[1] 

机构地区：[1]广州军区广州总医院药学部,广州510010

出　　处：《现代食品与药品杂志》2006年第2期25-27,共3页JOurnal of Modern Food and Pharmaceuticals

摘　　要：目的以RP-HPLC测定人血浆中盐酸二甲双胍的浓度。方法色谱柱为Diamosil C18（5μm，4．6mm×250mm），流动相为0．01mol·L^-1 KH2PO4 -006mol·L^-1 SDS-乙腈（34．25：34．25：31．5），流速为1．0ml·min^01，检测波长为233nm，内标为阿替洛尔。结果盐酸二甲双胍的线性范围为0．05-4．00μg·ml^-1，回归方程为Y=1．3397X＋0．0222（r=0．9999）；日内精密度为1．25％～6．14％，日间精密度为0．93％-6．43％。结论本方法操作简便，测定结果准确，重复性好，可用于盐酸二甲双胍人体药代动力学研究。Objective To establish a RP-HPLC for the determination of Metformin Hydrochloride in human plasma. Method The Diamosil C18 column （5 μm, 4.6 mm ×250 mm） was used with mobile phase of 0.01 mol·L^-1 KH2PO4 -0.006 mol· L^-1 SDS-acetonitrile （34.25： 34.25： 31.5）. The flow-rate was 1. 0 ml ·min^-1. The detection wavelength was 233 nm. Internal standard was Atenolol. Result The linear range of the calibration was （0.05 - 4, 00） μg·ml^-1 （ Y = 1. 3397X ＋ 0. 0222, r = 0, 9999）. Within-day RSD was 1. 25% -6.14%. Between-day RSD was 0.93% % - 6.43%. Conclusion The method is simple, accurate and was used in the phamarmacokinetic study of Mettformin Hydrochloride.

关 键 词：盐酸二甲双胍 反相高效液相色谱法 血浆浓度 

分 类 号：R927.11[医药卫生—药学]