洋葱伯克霍尔德氏菌L68双加氧酶区基因克隆及序列分析  

Cloning and Sequence Analysis of Dioxygenase Region of Burkholderia cepacia L68

作　　者：吕鹏[1] 张长铠[1] 

机构地区：[1]山东大学生命科学学院微生物技术国家重点实验室,山东济南250100

出　　处：《生物技术》2006年第2期8-11,共4页Biotechnology

基　　金：国家自然科学基金资助项目(30370014)

摘　　要：克隆洋葱伯克霍尔德氏菌L68双加氧酶区基因并对其进行序列分析。采用邻苯二酚喷洒方法从洋葱伯克霍尔德氏菌L68的基因文库中筛选到了1株含有双加氧酶区基因的重组子,其重组质粒命名为pB2k。重组质粒pB2k含有8030bp的L68基因片断,经BLAST比对,该片断含有11个与已报道的ORF相近的ORF。在该片断的5’端,ORF1和ORF2分别编码两个转移酶基因,tomA1t、omA2t、omA3和tomA4编码酚羟化酶组份,tomA5编码氧化还原酶,phnT编码铁氧还蛋白,phnE编码邻苯二酚2,3-双加氧酶,ORF3编码未知功能蛋白,phnG编码部分2-羟粘糠酸半醛脱氢酶。A yellow clone containing functional catechol 2,3 - dioxygenase gene was obtained from the genomic library of Burkho/der/a cepac/a L68 by catechol spray. Froro the positive clone, a recombinant plasmid with an 8 kb EcoR Ⅰ fragment of L68 was extracted and designated as pB2K. An 8 kb region of L68 contains the gene for catechol 2,3 - dioxygenase was cloned and sequenced. The 8 kb region harbors genes encoding two transposase and inactivated derivatives, four phenol hydroxylase components, an oxidoreductase, a chloroplast - like ferredoxin, a catechol 2,3 - dioxygenase, an ORF similar to protein of unknown function DUF336, and a 2 - hydroxymuconic semialdehyde dehydrogenase.

关 键 词：洋葱伯克霍尔德氏菌 双加氧酶 基因克隆 序列分析 

分 类 号：Q933[生物学—微生物学] Q785