苛求芽孢杆菌基因组DNA提取方法的比较  

Comparison of Methods to Prepare Genomic DNA from Baccilus fastidious

作　　者：赵运胜[1] 卜友泉[1] 张宏娟[2] 廖飞[1] 

机构地区：[1]基础医学院生物化学与分子生物学教研室,重庆医科大学,重庆400016 [2]检验系临床微生物学教研室,重庆医科大学,重庆400016

出　　处：《生物技术》2006年第2期41-42,共2页Biotechnology

基　　金：国家自然科学基金项目资助(30200266)

摘　　要：目的:比较不同方法提取苛求芽孢杆菌基因组DNA的差异。方法:用经典CTAB提取法、改进CTAB法(溶菌酶处理结合CTAB提取法)、UniQ柱吸附提取法制备苛求芽孢杆菌基因组DNA,比较产物完整性和用于PCR扩增的有效性。结果:三种方法制备基因组DNA纯度接近,但改进CTAB法产率最高,UniQ法产率最低。经典CTAB法和UniQ法提取基因组DNA易降解。三种方法所得基因组DNA用于PCR扩增效率接近。结论:溶菌酶裂解结合CTAB提取更适合制备苛求芽孢杆菌基因组DNA。Objeetives：To compare methods for the preparation of genomic DNA from Bacillus fastidious. Methods： Genonic DNA was extracted by classical CrAB method, modified CTAB method with the use of lysozyme and UniQ ion- exchange method, the quality and quantity of genomic DNA were compared. Results： The modified CTAB method showed the highest yield, while the UniQ ion- exchange method showed the lowest yield of genomic DNA. These three methods gave DNA preparation of comparable homogenecity and efficiiency as the template for PCR amplification. Conclusion： The modified CTAB method was better for preparing genomic DNA from Bacillus fastidious.

关 键 词：苛求芽孢杆菌 基因组DNA提取 改进CTAB法 

分 类 号：Q523[生物学—生物化学]