花生慢生根瘤菌的分离与鉴定  

Isolation and Identify of Bradyrhizobium Bacterium from Peanut

作　　者：朱剑光[1] 尉亚辉[1] 吴艺舟[1] 

机构地区：[1]西北大学生命科学学院教育部西部资源与现代生物技术重点实验室,陕西西安710069

出　　处：《生物技术》2006年第2期45-47,共3页Biotechnology

基　　金：陕西省自然科学基金资助项目(99JK122);陕西省教育厅专项科研资金(04JK133)

摘　　要：目的：从野生花生的新鲜根瘤中分离纯化花生慢生根瘤菌。方法：将野生型花生的新鲜根瘤灭菌后捣碎制成悬浮液，通过稀释涂布、划线和贴片三种方法，在YMA结晶紫和YMA刚果红选择培养基上逐步分离纯化得到单菌落。结果：三种方法均分离得到表面性状一致的单菌落，其中用稀释5^3-5^4倍的悬浮液划线分离的效果最好。所得菌株经理化性质和回接试验鉴定为慢生根瘤菌，从而为接着的花生根瘤菌及其结瘤基因的研究提供了基础。Objectives： To isolate the bradyrhizobium bacterium from peanut. Methods： Triturated fresh root nodules that were surface sterilized. Then, by spreading, streaking, and adhibiting, the bacterium was isolated from fresh suspend of wild peanut＇s root nodules. Results： Bacterium strains were isolated by all of three methods. And, the best result appeared when the fresh suspend was diluted by 5^3 -5^4 times sterilized water. Character and re- inoculation experiment indicated that the strains were Bradyrhizobium sp. （A rachis）, which provide a base for the coming research of peanut＇s nodulation gene.

关 键 词：慢生根瘤菌 花生 分离纯化 回接 PCR鉴定 

分 类 号：Q939[生物学—微生物学] Q93-331