胆固醇MIPs的合成及其免疫吸附性能评价和应用  

Syntheses of Cholesterol-MIPs and Their Absorption Properties and Applications

作　　者：吕斌[1] 江明[1] 石丹[1] 张江华[1] 梅素容[1] 朱业湘[1] 郝巧玲[1] 周宜开[1] 

机构地区：[1]华中科技大学同济公共卫生学院环境与健康教育部重点实验室,武汉430030

出　　处：《应用化学》2006年第4期353-356,共4页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金(30200061);湖北省卫生厅(JX1B031)资助项目

摘　　要：分别采用热和紫外光辐射引发合成胆固醇的非共价型M IPs,评价了不同M IPs的吸附特性,以合成的2种M IPs为免疫吸附剂,优化吸附和洗脱条件,对血液、蛋黄、牛奶等生物样品进行免疫吸附。结果表明,M IPs可有效萃取3种生物样品中的胆固醇,其中对血清的效果最好,蛋黄的效果最差。紫外光引发M IPs对胆固醇的回收率明显高于常规的C18柱萃取法。Non-covalent molecular imprinted polymers （MIP） of cholesterol were prepared from its reaction with methacrylic acid（MAA）, ethylene glycol dimethacrylate（EGDMA） and azobisisobutyronitrile（A1BN） v/a thermal and UV polymerization. The binding capability and selectivity of the different polymers for cholesterol were studied. The polymers were used as immunosorbents for the Selective enrichment of cholesterol in different biological samples（ serum, milk and yolk） under optimal loading, washing and eluting conditions. Resuhs show that MIPs can selectively recognize and separate cholesterol in all three biological samples. The rate of recovery of cholesterol from serum was the highest, while that from yolk was the lowest. Compared with C18 SPE, the UV initiated MIPs show higher recovery than C18 for cholesterol in the sample.

关 键 词：胆固醇 分子印迹聚合物 固相萃取 效率 生物样品 

分 类 号：O652.6[理学—分析化学] R446.1[理学—化学]