重组人生长激素对SW872脂肪细胞脂联素及其受体基因表达和蛋白分泌的影响  

Effects of Recombinant Human Growth Hormone on mRNA Expressions of Adiponectin and Its Receptors and Adiponectin Secretion in SW872 Adipocytes in Vitro

作　　者：包忠宪[1] 张龙江[1] 邓惠玲[1] 林汉华[2] 王宏伟[2] 

机构地区：[1]广东医学院附属深圳南山人民医院儿科,广东深圳518052 [2]华中科技大学同济医学院附属同济医院儿科,武汉430030

出　　处：《实用儿科临床杂志》2006年第8期465-466,484,共3页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金项目资助(30470645)

摘　　要：目的 观察重组人生长激素（rhGH）对脂肪细胞脂联素及其受体mRNA表达及蛋白分泌的影响，探讨GH诱导胰岛素抵抗的机制。方法体外培养、油酸诱导SW872前脂肪细胞分化为成熟脂肪细胞，加入rhGH干预；RT-PCR方法检测脂联素、脂联素受体1和2（AdipoR1，2）mRNA水平，ELISA方法检测细胞培养上清中脂联素蛋白含量。结果rhGH100～1000μg／L作用24h不同程度的降低SW872脂肪细胞脂联素及AdipoR1 mRNA的表达及脂联素的分泌，且rhGH浓度越高抑制作用越强；500μg／L rhGH作用4h，对脂联素及AdipoR1基因表达及脂联素的分泌无影响；随作用时间的延长，rhGH抑制脂联素及AdipoR1 mRNA的表达和脂联素的分泌；rhGH对AdipoR2基因表达无影响。结论rhGH以剂量和时间相关的方式抑制SW872脂肪细胞脂联素及AdipoR1 mRNA的表达和脂联素蛋白的分泌。Objective To study the influence of recombinant human growth hormone（rhGH） on adiponeetin and its receptors gene expression and adiponectin secretion in SW872 fat cells. Methods SW872 preadipocytes were cultured and induced to differentiate oleic acid in vitro. The levels of adiponectin and its receptors（ AdipoR1 and AdipoR2） mRNA were measured by semi - quantitative RT- PCR, The concentration of adiponectin in supernatant of cultured cells was assayed by ELISA method. Results The cells incubated for 24 hours with rhGH（ 100 - 1000 μg/L, respectively） decreased, as well as adiponectin, AdipoR1 mRNA and adiponectin secretion. The cells treated for 4 hours with rhGH（500 μg/L） represented no change in adiponectin mRNA, AdipoR1 mRNA and adiponectin secretion. However, the inhibition effect of rhGH were observed at 8 hours post - treatment. AdipoR2 mRNA expression was not influenced by rhGH. Conclusion It is suggested that rhGH can inhibit adiponectin mRNA,AdipoR1 mRNA expression and adiponectin secretion in SW872 fat cells in a time and dose- related manner.

关 键 词：SW872脂细胞 重组人生长激素 脂联素 脂联素受体 

分 类 号：R96[医药卫生—药理学]