△~6-脂肪酸脱饱和酶基因种子特异表达载体的构建  

Construction of Seed-specific Expression Vector of Δ~6-Fatty Acid Desaturase Gene

作　　者：冷虹[1] 李加纳[1] 陆合[1,2] 柴友荣[1] 殷家明[1] 

机构地区：[1]重庆市油菜工程技术研究中心,西南大学农学与生命科学学院 [2]重庆医科大学基础医学院微生物与免疫学教研室,重庆400000

出　　处：《中国农学通报》2006年第4期66-70,共5页Chinese Agricultural Science Bulletin

基　　金：农业部948项目"油菜特异资源和育种新技术的引进;改良及创新研究"(2003-Q04)资助。

摘　　要：以napA基因序列设计引物,以甘蓝型油菜中油821基因组总DNA为模板,通过PCR扩增,克隆了种子特异表达napin启动子。序列分析表明,试验得到扩增片段长1148bp,含有其它napin启动子序列共有的高度保守序列。将扩增序列与真菌匍枝根霉Δ6-脂肪酸脱氢酶基因RnD6D连接,构建了RnD6D的种子特异表达载体pCNR,为获得高产γ-亚麻酸的转基因油菜奠定了基础。By designing a pair of specific primers complementary to napA sequence, the seed - specific expression promoter of napin gene was cloned from Brassica napus Zhongyou 821 genomic DNA via polymerase chain reaction. The sequence analysis indicated that the cloned fragment contained 1148 nucleotides and possessed highly conservative sequences present in other napin promoters. The expected expression vector carrying △^6-fatty acid desaturase gene which was isolated from Rhizopus stolonifer under the control of Napin promoter was constructed, thus making possible the subsequent production of transgenic rapeseed with highγ-linolenic acid content.

关 键 词：油菜 种子特异启动子 △^6-脂肪酸脱氢酶基因 植物表达载体 

分 类 号：Q943[生物学—植物学] Q78