小鼠白细胞介素-18的克隆及真核表达质粒的构建  

Cloning of Mouse Interleukin-18 and Construction of Eukaryotic Expression Plasmid

作　　者：陈勇[1] 赵雪花[1] 何丽娅[1] 成彩莲[1] 夏瑞明[1] 朱汉荣[1] 王海燕[1] 

机构地区：[1]武汉科技大学医学院,湖北武汉430080

出　　处：《武汉科技大学学报》2006年第2期200-202,共3页Journal of Wuhan University of Science and Technology

摘　　要：克隆小鼠白细胞介素-18(m IL-18),并与真核表达质粒pcDNA3.1/H isB重组,构建真核表达重组质粒。采用RT-PCR,从小鼠肝细胞中扩增IL-18的全长cDNA。经H indⅢ,EcoR I双酶切,将该cDNA片断插入表达载体pcDNA3.1/H isB。通过酶切、PCR及测序对重组体进行鉴定。经鉴定,重组质粒构建正确,成功构建了真核表达载体pcDNA3.1/m IL-18,为下一步进行IL-18的功能研究奠定了基础。The cDNA of mouse interleukin-18 （IL-18） was cloned and recombined with plasmid pcDNA3.1/ His B to construct an eukaryotic expression plasmid. The cDNA of mouse IL-18 was amplified from the liver cells by RT-PCR. Having being digested by Hind Ⅲ and EcoR Ⅰ, this cDNA fragment was inserted into expression vector pcDNA3.1/His B. The recombinant plasmid was identified by restriction enzyme digest, PCR and sequencing. The identification has confirmed that the eukaryotic expression plasmid pcDNA3.1/ mIL-18 had been successfully constructed, which has provided foundation for further research.

关 键 词：白细胞介素-18 RT-PCR 真核表达质粒 

分 类 号：R392.1[医药卫生—免疫学]