反相离子对高效液相色谱法测定大鼠缺血再灌注心肌ATP的含量  

Determination of ATP in the rat heart with ischemia reperfusion by ion-pair reversed-phase HPLC

作　　者：周玉娟[1] 刘福林[2] 崔秀彦[2] 

机构地区：[1]河北职工医学院药理教研室,保定0710001 [2]河北职工医学院附属医院心外科,保定0710001

出　　处：《军医进修学院学报》2006年第2期134-135,共2页Academic Journal of Pla Postgraduate Medical School

基　　金：保定市科技局资助项目(99F008)

摘　　要：目的：研究大鼠心肌缺血再灌注损伤后三磷酸腺苷（ATP）的含量的变化.方法：应用反相离子对高效液相色谱法（HPLC）分离测定,流动相为 0.1 mmol/L KH2PO4＋8 mmol/L 氢氧化四丁铵,检测波长259nm.结果：ATP标准液与峰面积呈良好的线性关系（r=0.998）.重复测定不同浓度ATP标准液精密度（变异系数）1.91%～5.78%.回收率为97.25%～100.05%.结论：反相离子对高效液相色谱法可以准确测定大鼠心肌组织ATP的含量.Objectlve：To study the change alteration of ATP in the rat heart with ischemia reperfusion injury by Ion-pair reversed-phase HPLC. Methods： Ion-pair reversed-phase HPLC methods was developed for determination of ATP. The solvent consist of 0. 1 mmol · L^-1 KH2PO4 ; 8 mmol · L^-1 tetrabutylammonium hydrogen. The separation was monitored at 259nm. Results：The calibration curve showed good linearity （ r = 0. 998 ） . The coefficients of variation （ C. V） were estimated from repeated determinations of standard ATP. The intraassay C. V varied from 1.91% to 5.78%. The recovery was between 97.25% and 100.05%. Conclusions：This methods is precise,it can be used for determination of ATP in the rat heart.

关 键 词：色谱法 高压液相 心肌缺血 再灌注损伤 三磷酸腺苷 

分 类 号：R542.2[医药卫生—心血管疾病]