人脐血干细胞移植治疗大鼠脑缺血的实验研究  

Experimental study of human umbilical cord blood cells transplantation for treatment of cerebral ischemia in rats

作　　者：潘凤华[1] 李晓波[1] 丁新生[1] 张炜民[1] 张智弘[1] 邓小萱[1] 姚娟[1] 

机构地区：[1]南京医科大学附属第一医院神经内科,210029

出　　处：《临床神经病学杂志》2006年第2期121-123,共3页Journal of Clinical Neurology

摘　　要：目的研究人脐血干细胞(HUCBCs)移植治疗脑缺血大鼠的疗效及HUCBCs在缺血大鼠脑内的状况。方法采集足月新生儿脐带血60～100ml,分离出其中的单个核细胞,体外培养并予5溴脱氧嘧啶尿苷(Brdu)标记48h。采用线栓法制作大鼠脑缺血再灌注模型,1d后将3×106HUCBCs经缺血侧侧脑室注射入大鼠脑内。在移植后不同时间对大鼠进行神经损害严重程度评分(NSS),用免疫组化技术观察移植后的HUCBCs的存活、迁徙、分化状况。结果HUCBCs在体外具有增殖能力;移植组大鼠自移植后3周起其NSS显著低于对照组(均P<0.05);移植后2周、4周、6周脑组织切片中均可见Brdu染色阳性细胞,缺血侧明显多于对侧(均P<0.05),移植后4周、6周明显多于移植后2周(均P<0.05);移植组各时间点脑组织切片中均可见神经巢蛋白阳性细胞;植入的HUCBCs在大鼠脑内能向损伤区域迁徙并能分化为星形胶质细胞、少突胶质细胞和神经元。结论HUCBCs能在缺血大鼠脑内存活、迁徙和分化,并能改善其神经功能。HUCBCs移植可作为脑梗死的有效治疗手段。Objective To explore the effect of human umbilical cord blood cells （HUCBCs） transplantation to treat cerebral ischemia of rats and the status of transplanted HUCBCs in the ischemic brain tissue of these rats. Methods The mononudeareells abstracted from 60 - 100 ml of cord blood of full-term babies were cultured in vitro and marked with 5-bromodeoxyuridine （ Brdu ） （ 5 μmol/L） for 2 days. The middle cerebral artery occlusion rat models were made and the HUCBCs （3 × 10^6 ） were transplanted into the lateral ventricular 1 day later. Neurological severity scores （NSS） tests were undertaken at different time point after transplantation, and iimmunohistochemistry method was used to check the migration and differentiation of HUCBCs. Results The HUCBCs had the capacity of proliferation in vitro and were induced to differentiate into astrocytes, oligodendrocytes and neurons in vivo. 3 weeks later, the neurological function of rats that received transplantation recovered much better than the rats without transplantation, as evidenced by NSS （all P 〈 0. 05 ）. Within the brain tissues, Brdu reactive HUCBCs were distributed throughout the lesions in recipient rats 2, 4 and 6 weeks after transplantation. The vast majority of cells localized in the ischemic hemisphere and few cells were observed in the contralateral hemisphere （ all P 〈 0. 05 ）. More Brdu reactive cells were found in the brain tissues at 4 and 6 weeks than at 2 weeks （ all P 〈 0. 05 ）. Nestin reactive cells were found within the lesions at all observing time point after transplantation. Conclusions The HUCBCs may survive, migrate and differentiate in the ischemic brain, and ameliorate neurologic function of rat. HUCBCs transplantation may provide a novel approach to treat stroke.

关 键 词：人脐血干细胞 移植 脑缺血 

分 类 号：R651[医药卫生—外科学]