维生素E对环磷酰胺致小鼠睾丸损伤的保护作用  

Antagonistic Effects of Vitamin E on the Testicular Injury by Cyclophosphamide in Mice

作　　者：李杜娟[1] 徐正顺[1] 张朝晖[1] 黄庆玉[2] 

机构地区：[1]河南科技大学医学院病理学教研室,河南洛阳471003 [2]泰山医学院病理学教研室,山东泰安271000

出　　处：《中华男科学杂志》2006年第4期318-322,共5页National Journal of Andrology

摘　　要：目的:观察维生素E对环磷酰胺(CP)致小鼠睾丸损伤的保护作用,并探讨其相关机制。方法:随机将50只小鼠均分成CP组、低剂量维生素E组(低剂量组)、中剂量维生素E组(中剂量组)、高剂量维生素E组(高剂量组)、正常对照组(对照组)。CP组和各维生素E组小鼠经口灌胃5 mg/(kg.d)CP,低、中、高剂量组小鼠每天CP处理4 h后给予皮下注射:30、50、70 mg/(kg.d)维生素E,对照组小鼠经口灌胃等量生理盐水。各组均连续处理28d后,检测血清卵泡刺激素(FSH)、黄体生成素(LH)、睾酮(T)水平,睾丸组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSHPx)、过氧化氢酶(CAT)活性以及丙二醛(MDA)含量,同时观察睾丸组织结构及超微结构。结果:中、高剂量组血清FSH、LH、T水平,以及SOD、GSHPx、CAT活性明显高于CP组(P<0.05或P<0.01),MDA含量明显低于CP组(P<0.01),睾丸生精小管结构规则,生精细胞层次较多,核膜清晰完整,线粒体、粗面内质网等无异常。结论:维生素E对CP致睾丸损伤有明显的保护作用,其作用可能与维生素E清除氧自由基、抗氧化作用及促进腺垂体促性腺激素的释放等机制有关。Objective： To observe the protective effects of vitamin E on the testicular injury by cyclophosphamide in mice, and the correlative mechanism. Methods： Fifty sexually mature male mice were randomly divided into five groups： the cyclophosphamide group（the CP group）, the low-dose vitamin E group （the low-dose group）, the middle-dose vitamin E group （the middle-dose group）, the high-dose vitamin E group （the high-dose group）, the matched control group （the control group）. The first four groups were given cyclophosphamide by garage at a dose of 5 mg/（ kg · d）. The low-dose group, the middle-dose group and the high-dose group were given vitamin E by subcutaneous injection at doses of 30 mg/（ kg · d） , 50 mg/（ kg · d） , 70 mg/（ kg · d） after 4 h of cyclophosphamide treatment. The control group was garaged with equivalent normal saline. The treatment period for all groups was 28 days. The level of plasma FSH, LH, T and the activity of testicular SOD, GSHPx, CAT and the level of testicular MDA were detected. The histological structure and the ultrastructure of the testis were examined by light microscope and electron microscope. Resuits： As compared with the CP group, the plasma FSH, LH, T level and the SOD, GSHPx, CAT activity in the middle-dose group and the high-dose group were higher（ P 〈 0.05, P 〈 0.01 ）, MDA level significantly lower（ P 〈 0.01 ）. The histological structure and the ultrastructure of the testis were in the normal range. Conclusion ： Vitamin E has protective effects on the testicular injury by cyclphophamide in mice. The possible mechanism of vitamin E may be its scavenging free radical and antioxidant effects, as well as it may have some stimulatory effects on gonadotrophin releasing of pituitary anterior lobe.

关 键 词：维生素E 环磷酰胺 睾九损伤 自由基 性腺 氧化应激 小鼠 

分 类 号：R96[医药卫生—药理学]