神经干细胞应用于组织工程化人工神经的实验研究  

Neuronal stem cells in facial nerve tissue engineering

作　　者：郭宝凤[1] 董明敏[1] 任秀花[2] 

机构地区：[1]郑州大学第一附属医院耳鼻咽喉科 [2]郑州大学基础医学院解剖教研室,河南郑州450052

出　　处：《中国耳鼻咽喉头颈外科》2006年第3期165-168,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：国家教育部"十五""211"工程重点学科"肿瘤与生物工程"资助项目[教重字(2002)第2号]

摘　　要：目的探讨神经干细胞(neuronalstemcells,NSCs)应用于组织工程化人工神经修复兔10mm长面神经缺损的效果。方法日本大耳白兔36只,随机分为3组,每组12只。A组:壳聚糖管加胶原蛋白海绵加神经生长因子(nervegrowthfactor,NGF)加NSCs;B组:壳聚糖管加胶原蛋白海绵加NGF;C组:自体神经移植。术后12周,进行系列神经电生理检测、神经组织学观察、BrdU和S100免疫组织化学检测等检查。结果术后12周,A组的各项检测指标均优于B组(P<0.05),与C组间的差异无显著性(P>0.05)。结论NSCs可作为周围神经组织工程中的种子细胞,并应用于人工神经修复面神经缺损。OBJECTIVE To evaluate the effectiveness of applying neuronal stem cells to bridging a 10mm long facial nerve defect in rabbits using a tissue engineered artificial nerve. METHODS Thirty-six Japanese white rabbits were randomly divided into three groups of 12. Group A： chitosan guidance channels with collagen protein sponge were filled with neuronal stem cells and NGF; Group B： chitosan guidance channels with collagen protein sponge were filled with NGF; Group C： autograft nerve. A series of examinations were performed, including electrophsiological methods, histological staining of nerve, Brdu and S100 immunohistochemical staining, at 12 weeks after operation. RESULTS At 12 weeks after operation, all examination results from Group A were better than from Group B （P〈0.05）, and there was no significant difference between Groups A and C（P〉0.05）. CONCLUSION Neuronal stem cells can be used as seeding cells in peripheral nerve tissue engineering for repairing 10mm facial nerve defects.

关 键 词：动物 实验 面神经 神经再生 神经生长因子 组织工程 

分 类 号：R651.3[医药卫生—外科学] R318.0[医药卫生—临床医学]