系统性白念珠菌感染小鼠IL-12和IL-23的表达  

The expression of IL-12 and IL-23 in mice with systemic Candida albicans infection

作　　者：李家文[1] 曹菲[1] 谭志建[1] 丁娟[1] 张德美[1] 邬炎卿[1] 曾敬思[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院皮肤科,武汉430022

出　　处：《中国麻风皮肤病杂志》2006年第4期270-273,共4页China Journal of Leprosy and Skin Diseases

基　　金：湖北省卫生厅科学基金资助项目(JX1B048)

摘　　要：目的了解系统性白念珠菌感染小鼠IL-12和IL-23的表达特征,探讨IL-23在抗白念珠菌系统感染中的作用,并重新认识和评价IL-12的作用。方法通过尾静脉接种白念珠菌建立小鼠白念珠菌系统感染模型,观察小鼠肾脏的病理变化,RT-PCR法检测小鼠肾脏IL-23及IL-12mRNA的相对表达水平,平皿稀释法检测肾脏内菌落形成单位(CFU)。结果IL-12mRNA表达水平在初次感染后第1、3天明显升高(P<0.05),感染后第7天基本正常。IL-23mRNA水平在初次感染后的第1天无明显改变(P>0.05),感染后第3、7天明显升高(P<0.05),而再次感染中其mRNA始终呈现高表达(P<0.05)。结论IL-12和IL-23均参与I型免疫反应,有效抵御白念珠菌系统感染,IL-12可能在炎症反应的早期发挥重要作用,而IL-23可能在炎症反应的后期发挥重要作用。Objective： To measure the expression of IL- 23 and IL - 12 in mice with disseminated eandidiasis, in order to understand the role of IL - 23 in the anti - systemic Candida albicans infection. Methods： The model of systemic Candida albicans infection was established by injection of the pathogenie fungi into the tail - vein of mouse to observe the pathological changes in the kidney of the mouse. RT - PCR was applied to detect the expression of IL -23 mRNA and IL- 12 mRNA in the kidney. Colony forming unit （CFU） of the infected kidney was determined with the plating dilution methods. Results： The level of IL- 12 mRNA was evidently higher than that of the normal controls on day 1 and day 3 （ P 〈 0.05）, and gradually returned to normal on day 7. In the primary infection the level of IL - 23 mRNA started to rise on day 3 and day 7, while the expression of IL - 23 mRNA rose to a high level from the beginning and persisted for the whole process, in the secondary infection （P〈0.05）. Conclusion： Both IL- 12 and IL - 23 are involved in type - I immune response and resist effectively against systemic Candida albicans infection. It is postulated that IL- 12 may play an important role in the early phase of the inflammation, while IL-23 lakes effect in the late phase.

关 键 词：系统性念珠菌感染 IL-23 IL-12 动物实验 

分 类 号：R519.3[医药卫生—内科学]