丹参酮ⅡA抑制血管紧张素Ⅱ诱导的心肌细胞凋亡与肥大  

The Effect of Tanshinone Ⅱ A on Apoptosis and Hypertrophy in Cardiomyocytes Induced by Angiotensin Ⅱ

作　　者：刘继军[1] 张宏考[1] 谢辉[1] 

机构地区：[1]郧阳医学院附属人民医院心内科,湖北十堰442000

出　　处：《高血压杂志》2006年第4期286-289,共4页Chinese Journal of Hypertension

基　　金：湖北省科技攻关课题(2002AA301C29)

摘　　要：目的研究丹参酮ⅡA(TSN)对血管紧张素Ⅱ(AngⅡ)诱导的乳鼠心肌细胞肥大、凋亡的影响。方法培养乳鼠心肌细胞分别暴露于AngⅡ(10-6)、TSN(10-6、AngⅡ(10-6mol/L)+TSN(10-5mol/L)36 h,检测心肌细胞的凋亡蛋白p53的表达、心肌细胞直径和3H-亮氨酸掺入率。结果TSN可抑制AngⅡ所诱导的心肌细胞的凋亡蛋白p53(AngⅡ+TAN:99 890±338比AngⅡ:389 712±270,P<0.05),抑制心肌细胞直径[(AngⅡ+TAN:21.3±2.5比AngⅡ:28.5±3.8)μm,P<0.05]和3H-亮氨酸掺入率[(AngⅡ+TAN:1 302±96比AngⅡ:1 900±100)计数/min,P<0.05]。结论TSN可抑制AngⅡ所诱导的心肌细胞的凋亡和肥大。Objective To study the effect of the effect of tanshinone Ⅱ A （TSN） on hypertrophy and apoptosis of cardiomyocytes induced by Ang Ⅱ. Methods The cultured neonatal rat cardiomyocytes （NRCs） were exposed to Ang Ⅱ （10^-6 mol/L）, TSN（10^-5 mol/L）and Ang Ⅱ （10^-6 mol/L）＋TSN （10^-5 mol/L） respectively in free-serum medium for 36 h. The expression level of apoptoic protein p53, the cell size and s H-Leucine incorporation were investigated. Results As compared with control, the apoptoic protein p53 expression level（Ang Ⅱ ＋TAN：99 890±338 vs Ang Ⅱ ：389 712±270, P〈0.05）, the cell size（Ang Ⅱ＋TAN：21.3±2. 5 vs Ang Ⅱ：28. 5±3.8 μm, P〈0. 05）and protein synthesis rate of cardinmyocytes（Ang Ⅱ ＋TAN： 1 302±96 vs Ang Ⅱ： 1 900±100 cpm, P〈0.05） were increased significantly after Ang Ⅱ treatment（P〈0. 05）. TSN inhibited these effects induced by AngⅡ （P〈0. 05）. Conclusion TSN may prevent cardiomyocytes hypertrophy and apoptosis induced by Ang Ⅱ.

关 键 词：丹参酮ⅡA 血管紧张素Ⅱ 心肌细胞 肥大 凋亡 P53 

分 类 号：R285[医药卫生—中药学]