光系统II核心复合物的稳态荧光光谱(英文)  

Steady State Fluorescence Spectroscopy of the Photosystem II Core Complex

作　　者：蔡霞[1] 王水才[2] 贺俊芳[2] 刘晓[1] 彭菊芳[2] 匡廷云[3] 

机构地区：[1]西北大学生命科学学院,西安710069 [2]中国科学院西安光学精密机械研究所瞬态光学技术国家重点实验室,西安710068 [3]中国科学院植物研究所光合作用研究中心,北京100093

出　　处：《植物生理与分子生物学学报》2006年第2期169-174,共6页Journal Of Plant Physiology and Molecular Biology

基　　金：国家重点基础研究发展规划项目(No.G1998010100)资助。~~

摘　　要：在 83K 和 160K 两个温度下,通过激发波长对荧光发射谱的影响研究了光系统II中核心复合物的荧光光谱特性。用不同波长的光激发,核心复合物的发射谱的最大发射峰值不变,用 480、489、495 和 507nm 的光分别激发核心复合物,其光谱最大峰值处的荧光强度随不同激发波长下β-胡萝卜素分子的吸收强度的增大而降低,在长波长区域光谱的变化依赖于首先被激发的色素分子。所以,激发波长的不同影响着核心复合物中能量传递的途径。通过高斯解析,分析出核心复合物中至少存在有 7组叶绿素a组分,它们是Chla660,Chla670,Chla680,Chla682,Chla684,Chla687和Chla690。Spectroscopic properties within the core complex of photosystem Ⅱ were investigated by studying the influence of the wavelength of excitation on the fluorescence emission spectrum. At two temperatures, when the core complex of PSⅡ isolated from spinach was excited at six different excitation wavelengths ranging from 436 nm to 520 nm, there is no difference in the maxima of the emission spectra of the core complex, and when the core complex was excited at 480, 489, 495 and 507 nm respectively, fluorescence intensities of maxima decrease with increasing of the absorbance of the β-carotene molecules at the four excitation wavelengths. The extent of change of the shoulder of the spectra beyond 700 nm depends on the kind of pigment molecule excited. The excitation wavelength can influence the way of energy transfer in the core complex of photosystem Ⅱ. By Gaussian deconvolution analysis, at least seven groups of chlorophyll a molecules were discovered. They are Ch1 a660, Ch1 a670, Ch1 a680, Ch1 a682, Ch1 a684, Ch1 a687 and Ch1 a690.

关 键 词：光系统Ⅱ核心复合物 光谱特性 温度 激发波长 

分 类 号：Q945.11[生物学—植物学]