丹皮总甙和白芍总甙对自由基清除作用的影响  

THE SCAVENGING ACTIVITIES OF TGM AND TGP ON FREE RADICALS

作　　者：高本波[1] 戴俐明[1] 徐叔云[1] 

机构地区：[1]安徽医科大学临床药理研究所

出　　处：《潍坊医学院学报》1996年第1期43-46,共4页Acta Academiae Medicinae Weifang

摘　　要：利用核黄素与光作用产生Ｏ２，Ｆｅｎｔｏｎ反应产生ＯＨ·，以及Ｈ２Ｏ２引起的红细胞氧化溶血的方法，研究了丹皮总甙（ＴＧＭ）和白芍总甙（ＴＧＰ）对Ｏ２及ＯＨ·的清除作用和对溶血反应的影响。结果ＴＧＭ和ＴＧＰ对Ｏ２产生５０％清除率时的浓度分别为２．３１ｍｇ／Ｌ和２．６４ｍｇ／Ｌ，对ＯＨ·产生５０％清除率时的浓度分别为２５．６９ｍｇ／Ｌ和１１７．９６ｍｇ／Ｌ，抑制Ｈ２Ｏ２引起溶血反应的ＩＣ５０分别是０．９４ｍｇ／Ｌ和１．４１ｍｇ／Ｌ。结果表明ＴＧＭ和ＴＧＰ对自由基有清除作用，并能抑制Ｈ２Ｏ２引起的溶血反应，且ＴＧＭ的作用强于ＴＧＰ。The models of simplified O2-generating system, OH·-generating system and oxidative hemolysis induced by hydrogen peroxide were used to study the O2,OH·scavenging activity and inhibitory effects on oxidative hemolysis of total glucosides of Moutan Corter(TGM) and total Glucosides of Paeony Lactiflora (TOP) in this paper.The results shows that TGM and TGP can scavenge O2 and OH.; IC50of scavenging O2 are 2.31 and 2.64mg/L respectively, IC50 of scavenging OH are 25.69 and 117.96mg/L respectively. The IC50 of inhibiting oxidative hemolysis are 0.94 and 1.41mg/L respectively. The results suggest that TGM and TGP have the capacity to scavenge O2 and OH·,and can inhibit oxidative hemolysis induced by H202. The radical scavenging activity of TGM,in comparison with that of TGP,was more effective.

关 键 词：丹皮总甙 白芍总甙 超氧阴离子 羟自由基 

分 类 号：R931.71[医药卫生—生药学]