病毒性心肌炎心肌超微结构及细胞凋亡的电镜观察  

Electron microscope observation of myocardial ultrastructure and cardiomyocyte apoptosis in viral myocarditis

作　　者：张松[1] 杨春山[1] 葛均波[1] 饶邦复[1] 

机构地区：[1]复旦大学附属中山医院心血管病研究所,上海200032

出　　处：《中国心血管杂志》2006年第2期81-82,95,共3页Chinese Journal of Cardiovascular Medicine

摘　　要：目的 研究病毒性心肌炎（VMC）小鼠心肌超微结构改变及细胞凋亡的形态学变化。方法 本实验在接种柯萨奇病毒B3（CVB3）建立VMC动物模型的基础上，用光学显微镜及电子显微镜观察心肌细胞的病变及心肌细胞凋亡。结果 研究发现实验组小鼠在接种病毒5d后光镜或电镜下可见心肌病变及炎细胞浸润，7～9d病变达高峰，35d时病变基本恢复。VMC小鼠在接种病毒后7-9d，电镜下可见心肌细胞呈凋亡样改变，并可见凋亡小体。结论 实验组小鼠在接种CVB3后可引起VMC，VMC中存在异常的心肌细胞凋亡现象。Objective To study ultrastructure changes of myocardium and morphic changes of cardiomyocyte apoptosis in mice with viral my ocarditis（VMC）. Methods Animal model of VMC were established by the way of coxsackie virus B3 （CVB3） inoculation, then the changes and apoptosis of cardiomyocyte were observation with microscope and electron microscope. Results The myocardial changes and inflammatory cell infiltration were found with microscope and electron microscope from 5 days after vircts inoculation in experimental mice, the peak changes were at 7- 9 days, and were almost recoverd at 35 days. Apoptotic changes and apoptotic bodies were found by electron microscope at 7-9 days after vircts inoculation in mice with VMC. Conclusion VMC can be caused in mice after CVB3 inoculation, abnormal cardiomyocyte apoptosis can be found in VMC.

关 键 词：病毒性心肌炎 凋亡 电子显微镜 

分 类 号：R542.21[医药卫生—心血管疾病]