维A酸对HL-60细胞端粒酶活性的影响  

Effect of All-trans Retinoic Acid on Telomerase Activity in HL-60 Cell Line

作　　者：李四强[1] 侯忠赤[2] 宫超[1] 刘伯英[1] 田嘉玲[1] 

机构地区：[1]解放军252医院血液科,河北保定071000 [2]北京大学医学部生理学系,北京100083

出　　处：《华北国防医药》2006年第2期85-86,共2页Medical Journal of Beijing Military Region

摘　　要：目的:探讨维A酸(ATRA)诱导HL-60细胞增殖过程中端粒酶活性的变化。方法:将不同浓度(10-6、10-7及10-8M)的ATRA处理体外培养的HL-60细胞,采用四甲基偶氮唑盐比色法(MTT)测定其细胞增殖情况,培养72 h时用TRAP-ELISA法检测端粒酶活性的变化。结果:维A酸呈时间-剂量依赖方式抑制HL-60细胞增殖,与对照组比较,实验组端粒酶活性均下降,其中10-6M及10-7M两组差异显著(P<0.05,P<0.01),而10-8M组无显著差异(P>0.05)。结论:维A酸能够抑制HL-60细胞增殖,该过程可能与其下调细胞端粒酶的活性有关。Objective ：To explore the change in telomerase activity during proliferation of HL-60 cells induced by all-trans retinoic acid （ATRA）. Methods： HL-60 cells were cultured in vitro with ATRA in different concentrations for different period of time. Cell viability of proliferation was assessed with MTT assay. The telomerase activity was determined with TRAP-ELISA technique 72 hours after the culture. Results： The proliferation of HL-60 cells was greatly inhibited by ATRA in a time-dosage-dependant manner. The telomerase activity was decreased after ATRA;there was a significant difference between either the concentration of 10^-6M group or 10^7M group and the control,whereas the group of 10^-6M was not significantly different from the control. Conclusion ： ATRA could inhibit the proliferation of HL-60 cells in vitro, and this effect minght be associated with downregulation of telomerase activity by ATRA..

关 键 词：维A酸 HL-60细胞 端粒酶 白血病 

分 类 号：R979.19[医药卫生—药品]