运动性动情周期抑制雌性大鼠下丘脑谷氨酸与GnRHmRNA关系的探讨  

Relationship between Glu and GnRH mRNA in hypothalamus of exercise-induced estrous cycle inhibited female rats

作　　者：高丽[1] 郑陆[1] 沈东颖[1] 宁翠叶[1] 宋昶[1] 栾天峰[1] 

机构地区：[1]山东体育学院,山东济南250063

出　　处：《山东体育学院学报》2006年第1期63-66,共4页Journal of Shandong Sport University

摘　　要：2月龄雌性SD大鼠进行十周大强度跑台运动后出现运动性动情周期抑制现象,以模拟长期耐力运动训练后女运动员出现的运动性闭经现象,酶法测定下丘脑谷氨酸的含量,RT-PCR法测定大鼠下丘脑GnRH mRNA表达。结果显示,与对照组相比,十周大强度跑台运动致运动性动情周期抑制大鼠血清雌、孕激素水平降低,下丘脑谷氨酸含量降低,下丘脑GnRH mRNA的表达降低。提示十周大强度跑台运动引起下丘脑GnRH的转录功能降低是运动性动情周期抑制产生的重要原因,下丘脑谷氨酸含量的降低可能是影响下丘脑GnRH转录功能的重要因素。2 - month female SD rats were carried with high intensity training in treadmill for 10 weeks. After training, rats displayed that their estrous cycle were inhibited to simulate phenomena of athlete secondary amenorrhea. The content of glutamate in hypothalamus was analyzed by enzyme. The level of GnRH mRNA in hypothalamus was analyzed by RT - PCR. The results showed that high intensity exercise induced most of rots＇ estrons cycle inhibited. The level of estrogen and progesterone in high intensity group were decreased than that in control. The content of glutamate in hypothalamus was lower in high intensity group than that in control. And expression of GnRH mRNA in hypothalamus of high intensity group was reduced than that in control. Those results indicated that long- time high intensity exercise would affect transcription of GnRH in hypothalamus, which is the important cause that inhibited estrous cycle. And the decreasing content of glutamate in typothalamus was one of the important reasons on transcription of GnRH in hypothalamus.

关 键 词：运动性动情周期抑制 下丘脑 谷氨酸 GNRH 

分 类 号：G804.2[文化科学—运动人体科学]