^(125)IUdR对大鼠胶质瘤细胞靶点放疗的研究  

Study of C6 glioma DNA-targeting radio-therapy by Auger electron emitted from ^(125)Iudr in rats.

作　　者：罗林[1] 白刚[1] 游潮[2] 左频[1] 赵建华[3] 司马秀田[1] 袁红平[1] 范耀东[1] 

机构地区：[1]云南省肿瘤医院神经外科,昆明650118 [2]华西医科大学 [3]云南省第一人民医院

出　　处：《云南医药》2006年第2期100-104,共5页Medicine and Pharmacy of Yunnan

基　　金：云南省自然科学基金资助项目(NO.1999CO112M)

摘　　要：目的在建立C6/Wistar胶质瘤大鼠模型的基础上,研究125IUdR介导俄歇电子释放对大鼠C6胶质瘤DNA靶点放疗的作用及其机制。方法将Wistar大鼠分为实验组(20只)、对照组(20只)和空白组(10只)3组,前两组再分为5日处死和生存分析两个亚组。实验组在肿瘤增殖高峰期于肿瘤接种原位分次注入125IUdR,0.2mCi/10μl/次;对照组同法注入等摩尔浓度的127IUdR、5.6uM/10μl次;空白组同法注入同量生理盐水,10μl/次。行免疫组化染色及核仁组织区银染检查,了解125IUdR和127IUdR治疗后C6胶质瘤细胞的增殖动力学改变。结果1.大体病理改变;治疗5d后,实验组大鼠肿瘤直径和重量均明显小于对照组(P<0.05)。2.HE染色:对照组坏死区增大,其他变化不明显,实验组C6肿瘤细胞的核分裂像计数减少,细胞坏死区增大较对照组更明显,肿瘤细胞的凋亡现象也更为多见。3.AgNOR染色;实验组AgNOR计数和面积均明显低于对照组(P<0.01)。4.免疫组化染色:实验组PCNA阳性表达低于对照组(P<0.05)。结论应用125IUdR内放射治疗肿瘤,可以有效的抑制肿瘤细胞的增殖,已成为肿瘤治疗的一种新途径。Objective To investigate the action and mechanism of Auger electron emitting from ^125IUdR on DNA - targeting radiotherapy of C6 glioma in rat based on establishment of a C6/Wistar glioma model. Methods A total of 50 male Wistar rats were randomingly divided into three groups： experimental group （n= 20）, control group （n= 20） and sham group （n = 10） . The animals of two former groups were separted into two subgroups： 5 day sacrificing group and survival analysis group. Experimental group were infused intmcerebraUy with ^125IUdR per 8 hour during proliferation peak （0.2mCi.10μl^-1 .time^-1）, total dosage is 0.6mCi/rat. Control group were infused intracerebrally with ^125IUdR per 8 hour （5.6μm· 10μl^-1 .time^-1 ）, total dosage is 16.8um/rat. Sham group were infused with 10μl of 0.9% saline. PCNA immunohistochemistry and nucleolar organizer regions silver stain were performed. Results 1. Gross pathological examinations demonstrated tumor weight and diameter in experimental group were lower than that of control group （ P〈0.05） .2. HE stain showed that infraction area increased in control group, but the increasing infraction area was larger in experimental group. 3. AgNOR area and number in experimental group were lower than control group （ P〈 0.01） . 4. The PCNA expression level was significantly lower than that of control group （ P 〈 0.05） . Conclusion Using the glioma DNA - targeting innerradiotherapy by Auger electron emitted from 125 Iudr, it can inhibit effectively the multiplication of tumor cell, it has been a new way of the tumor therapy.

关 键 词：^125IUDR C6胶质瘤细胞 增殖动力学 俄歇电子 

分 类 号：R651[医药卫生—外科学]