空调生存环境对豚鼠红细胞膜脂质变化的影响  

Effect of Air - conditioning Environment on Erythrocyte Membrane Lipid in Guinea Pigs

作　　者：徐华建[1] 李甜[2] 李琳彬[3] 张传清[3] 

机构地区：[1]重庆医科大学附属第一医院检验科,重庆400016 [2]重庆医科大学医学检验系2001级 [3]重庆市中医院

出　　处：《华南国防医学杂志》2006年第2期5-7,共3页Military Medical Journal of South China

基　　金：重庆市卫生局基金项目(编号:04-2-156)

摘　　要：目的通过动物实验,研究空调环境对机体红细胞膜脂质成分的影响,为进一步研究空调环境对红细胞变形性的影响提供实验依据。方法采用豚鼠作为实验动物。实验分两组,每组12只。对照组:豚鼠在通风房间中自由饮水和进食,喂养15 天。实验组:豚鼠在温度为22℃空调房间内饲养15天。15天后,用心脏穿刺法采集豚鼠血液5ml,肝素抗凝,分离红细胞制备红细胞膜悬液。用UV265紫外分光光度计测定红细胞膜胆固醇及磷脂的含量。结果空调环境下豚鼠红细胞膜中胆固醇含量比正常对照组显著增高(P<0．01),而磷脂含量比对照组显著降低(P<0．01)且胆固醇／磷脂也有显著差异(P<0．01)。结论空调环境能改变红细胞膜的脂质成分,从而影响红细胞变形性。Objective To study the effect of air - conditioning environment on the erythrocyte membrane hpid of guinea pig in order to provide an evidence for studying the erythrocyte deformability in air - conditioning environment further. Methods Twenty-four guinea pigs were averaglly divided into two groups. The guinea pigs in the control group were fed with water and food in a well ventilated room for 15 days. The guinea pigs in the experimental group were fed with the same water and food in air - conditioning room in which the temperature was 22℃ for 15 days. Five milliliter blood derived from the heart of each guinea pig and heparin sodium was used as anticoagulant. The erythrocytes were separated and the susspension of the erythrocyte membrane was prepared. The cholesterol and phospholipids contents of the erythrocyte membrane were detected by UV256 ultraviolet spectrophotometer. Results The evel of cholesterol of the erythrocyte membrane significantly increased and the phospholipids significantly dereased in the experimental group compared with the control group （ P 〈0.01）. Conclusion The airconditioning environment can produce the change in the composition of erythrocyte membrane lipid and influence the erythrocyte deformability.

关 键 词：空调 环境 红细胞 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学]