乙型肝炎前S1抗原与HBV-DNA和HBeAg检测结果对比分析  

Comparative analysis on detection results of HBV preS1-Ag,HBV-DNA and HBeAg

作　　者：任瑞庆 陈寿云 陈炳辉[1] 陈婉婵 何雪环 

机构地区：[1]广东省东莞市石龙人民医院检验科,523321

出　　处：《国际检验医学杂志》2006年第4期300-302,共3页International Journal of Laboratory Medicine

摘　　要：目的对比分析检测HBV前S1抗原、HBV-DNA和HBeAg在乙型肝炎感染期主要模式组中的临床意义。方法用酶联免疫吸附法(ELISA)分别检测HBV前S1抗原和乙肝血清标志物“五项”,荧光定量聚合酶链反应(FQ-PCR)检测HBV-DNA,对308例检测结果进行统计分析。结果308例乙型肝炎患者血清标志物“五项”4种模式组即“HBsAg、HBeAg、HBcAb”阳性模式组、“HBsAg、HBeAb、HBcAb”阳性模式组、“HBsAg、HBeAg”阳性模式组和“HBsAg、HBcAb”阳性模式组。前S1抗原总阳性率为62.34%(192/308),HBV-DNA总阳性率为78.90%(243/308)。在HBe阳性模式组与HBeAg阴性/抗HBeAb阳性模式组对比,前S1抗原总阳性率分别为71.65%(139/194)和46.49%(53/114),阳性率差异具有统计学意义(χ2=18.30,P<0.01)。HBV-DNA阳性组和HBV-DNA阴性组前S1抗原阳性率分别为74.90%(182/243)和15.38%(10/65),阳性率差异具有统计学意义(χ2=74.85,P<0.01)。前S1抗原与HBeAg检测符合率为64.94%,与HBV-DNA检测符合率为76.95%。结论检测HBV前S1抗原阳性率在HBe阳性模式组中明显高于HBeAg阴性/抗HBeAb阳性模式组;前S1抗原与HBV-DNA和HBeAg检测有显著相关性和互补性;前S1抗原可作为检测HBV复制新的血清标志物。Objective To study the clinical significance of HBV preS1-Ag, HBV-DNA and HBeAg in main groups of HBV infection. Methods HBV preS1-Ag and hepatitis B serum markers individually were detected by ELISA,and HBV-DNA by fluorescence quantitative PCR, then the detection results of the 308 cases were analysed. Results The serum markers of the 308 cases infected by HBV presented four modes which were ＂HBsAg, HBeAg, HBeAb＂, ＂HBsAg, HBeAb, HBcAb＂,＂ HBsAg,HBeAg＂ ,＂ HBsAg, HBeAb＂. The total positive rate of preS1-Ag was 62. 34% （192/308）, and HBV-DNA was 78.90% （ 243/308 ）. Compared HBeAg （ ＋） groups with HBeAg （ - ）/anti-HBe （ ＋ ） groups, the corresponding positive rates of preS1-Ag were 71.65 % （ 139/194） and 46.49 % （53/114）, respectively. The difference of the two groups was significant （ χ^2 = 18. 30; P〈0. 01）. Compared HBV DNA（＋） groups with HBV DNA（- ） groups, the corresponding positive rate of preS1 were 74.90%（182/243） and 15.38%（10/65）, thus they had statistical differenees（χ^2 74.85; P〈0.01）. The detection concordance of preS1-Ag and HBeAg was 64.94%, while the concordance of preS1-Ag and HBV-DNA was 76. 95%. Conclusion The positive rate of HBV preS1-Ag detection in HBeAg （＋）groups is higher than others. Obvious correlation and eomplementation are found among preS1- Ag, HBV-DNA and HBeAg,so it is concluded that preS1-Ag could be a new serum marker for detecting the copy of HBV.

关 键 词：蛋白质前体 肝炎 乙型 DNA 病毒 肝炎e抗原 乙型 肝炎病毒 乙型 

分 类 号：R446.6[医药卫生—诊断学] R450[医药卫生—临床医学]