多胺对离体黄瓜子叶花芽构建的影响  

The Effect of Polyamines on the Floral Bud Construction of in vitro Cotyledons in Cucumis sativus (Cucurbitaceae)

作　　者：黄作喜[1] 唐正义[1] 谢寅峰[2] 段辉国[1] 卿东红[1] 

机构地区：[1]内江师范学院化学与生命科学系 [2]南京林业大学森林资源与环境学院,江苏南京210037

出　　处：《云南植物研究》2006年第2期194-198,共5页Acta Botanica Yunnanica

基　　金：四川省教育厅自然科学基金重点项目(No.2003A164)

摘　　要：研究了外源多胺对离体黄瓜(CucumissativusL.)子叶内在多胺含量和花芽构建的影响。在MS培养基中添加5×10-2mmol·L-1亚精胺(Spd)、精胺(Spm)能明显提高子叶内Spd含量和SpdPut值,子叶成花率也明显高于对照组,而添加5×10-2mmol·L-1Put、5mmol·L-1环己胺硫酸盐(CHAS)培养的结果则相反。表明子叶内Spd含量和SpdPut与成花率呈正相关关系。在添加5mmol·L-1D精氨酸(DArg)处理后,子叶内SpdPut高于Spd处理,但子叶成花率低于对照组,表明Put、Spd含量也影响离体子叶花芽的构建。在实验中添加CHAS处理后子叶内Spm含量高出对照组10倍,子叶成花率却为0,其它各处理与对照组的内源Spm变化基本一致,成花率却差别明显,表明子叶内Spm含量与黄瓜子叶花芽构建之间无明显相关性。The effects of exogenous polyamines on the levels of polyamines in the in vitro cotyledons and on the floral bud construction were examined in Cucumis sativus. Application of 5×10^-2 mmol·L^-1 spermidine （Spd） and spermine （Spm） in MS culture medium remarkably increases the levels of Spd and the ratio of Spd/putrescine （Put） in cotyledons and results in the rate of flora buds higher than that of control; however, apphcation of 5×10^-2 mmol·L^-1 Put or 5 mmol·L^-1 CHAS leads to opposite results, suggesting that the levels of Spd and ratio of Spd/Put in cotyledons are positive correlated with the rate of floral buds. Application of 5 mmol·L^-1 D-Arg in medium results in that the ratio of Spd/Put is higher than that treated with Spd and that rate of floral buds in cotyledons is lower than that of control, indicating that the levels of Put and Spd have effects on the construction of floral buds. Comparing with control, application of CHAS increases the levels of Spm in cotyledons 10 folds and decreases the rate of floral buds to zero. There is no significant difference in the levels of endogenous Spm between other treatments and control, except the rate of floral buds, meaning there is no correlation between the levels of Spm in cotyledons and the construction of floral buds in Cucumis sativus.

关 键 词：多胺 离体黄瓜子叶 花芽构建 

分 类 号：Q945.45[生物学—植物学] Q943.1