新疆地区大肠埃希菌和克雷伯菌中产超广谱β-内酰胺酶基因型的研究  

ESBLs′ Genotypes of Escherichia coli and Klebsiella spp Isolated from Xinjiang: A Study

作　　者：杜敏[1] 陈旭[1] 马尚柱[1] 马集云[1] 张淑兰[1] 杨启文[2] 徐英春[2] 

机构地区：[1]新疆兵团医院,新疆乌鲁木齐830002 [2]中国医学科学院北京协和医院,北京100730

出　　处：《中华医院感染学杂志》2006年第4期361-364,共4页Chinese Journal of Nosocomiology

基　　金：新疆兵团科研与技术开发基金项目

摘　　要：目的了解新疆地区3所医院产超广谱β-内酰胺酶(ESBLs)大肠埃希菌和克雷伯菌的耐药性及所产ESBLs的基因型特征。方法琼脂平皿对倍稀释法测10种抗菌药物的最低抑菌浓度(MICs);PCR通用引物扩增blaTEM、blaSHV、blaCTX-M基因后测序以确定基因亚型;对PCR未扩增出ESBLs基因的菌株采用等电聚焦电泳检测菌株所产β-内酰胺酶的等电点。结果测试菌株对氨曲南、头孢噻肟、头孢他啶的敏感率较低,对头孢吡肟、头孢哌酮/舒巴坦、头孢美唑、哌拉西林/他唑巴坦和美罗培南保持了相对较高的敏感率;55株菌主要携带blaCTX-M-1组基因和blaCTX-M-9组基因,另外blaSHV-12基因也占了一定比例。结论新疆地区3所医院产ESBLs的大肠埃希菌和克雷伯菌中有blaCTX-M-1组基因和blaCTX-M-9组基因的流行,blaSHV-12基因也在导致测试菌对β-内酰胺类药物耐药中起了一定作用。OBJECTIVE To investigate the resistant patterns of ESBLs-producing Escherichia coli and Klebsiella spp isolated from 3 hospitals in Xinjiang and to determine the genotypes of ESBLs. METHODS Using 2-fold agar dilution test to determine MICs of ten antibiotics. Using polymerase chain reaction and DNA sequencing to determine ESBLs genotype; using isoelectric focusing to determine the isoelectric points of β-lactamases produced by strains in which no ESBLs genes were amplified by PCR. RESULTS Strains had low susceptibility to aztreonam, cefotaxime and ceftazidime. The susceptibility to cefepime, cefoperazone/sulbactam, cefmetazole, piperacillin/tazobactam, and meropenem were relatively high. Fifty five strains mainly carried blaCTX-M-1 subgroup gene and blaCTX-M -9 subgroup gene, and blaSHV-12 gene were also detected. CONCLUSIONS blaCTX-M-1 Group gene and blaCTX-M-9 group gene are prevalent in three hospitals of Xinjiang, blaSHV-12 gene also plays a part role in leading to resistapce of E. coli and Klebsiella spp to β-1actams.

关 键 词：大肠埃希菌 克雷伯菌 ESBLs基因型 

分 类 号：R378.21[医药卫生—病原生物学]