枳黄方对急性酒精性肝病大鼠TNF-α和内毒素的影响  

The effect of zhihung decoction on levels of TNF-α and endotoxin in serum in rats'liver induced by alcohol

作　　者：张维丽[1] 潘玲[1] 胡胜军[1] 邱荣元[1] 杨玲[2] 彭汉光[2] 

机构地区：[1]华中科技大学同济医院2003级研究生班,湖北武汉430022 [2]华中科技大学同济医院中西医结合科

出　　处：《中西医结合肝病杂志》2006年第2期99-100,共2页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

摘　　要：目的:通过观察枳黄方对大鼠急性酒精性肝损伤肝组织TNF-α的变化及其血浆内毒素水平的改变,验证此方对大鼠急性酒精性肝病的防治作用。方法:将75只Wistar大鼠随机分为枳黄方组、模型组及正常组,采用免疫组化法测量肝组织内TNF-α值,用生化法检测血浆内毒素水平。结果:枳黄方组大鼠血浆内毒素水平高于正常组(P<0·05),但显著低于模型组(P<0·01),肝组织TNF-α和血清ALT水平高于正常组(P<0·01),但显著低于模型组(P<0·01)。并且血浆内毒素水平与肝组织TNF-α活性变化呈正相关(r=0·993,P<0·01)。结论:枳黄方合剂可显著降低急性酒精性肝病大鼠血液中内毒素水平及肝脏中TNF-α水平,对大鼠急性酒精性肝病有较好治疗作用。Objective： To investigate the levels of TNF-α and endotoxin in serum in rats to study the effect of Zhihuang Decoction （ZHF） on acute alcoholic liver disease. Methods： Seventy-five wistar rats were divide into treat group, medel group and normal group. The content of TNF-α in liver were assayed by immune-histochemistry, the plasma content of endotoxin were assayed by biochemistry. Results： The levels of endotoxin of the treat group were higher than normal group （P〈 0.01）. The contents of TNF-α and ALT＇ s levels also were higher than medel group （P〈0.01）. and significantly higher than the medel group （P 〈 0.01 ）. The plasma level of endotoxin was positively correlated to TNF-α（r = 0.093, P 〈 0.01 ）. Conclusion： ZHF can decline significantly levels of TNF-α and endotoxin on serum in rats＇ liver induced by alcohol, can treat and prevent acute alcoholic liver disease.

关 键 词：枳黄方/药效学 急性酒精性肝病 内毒素 肿瘤坏死因子-Α 大鼠 

分 类 号：R285.5[医药卫生—中药学]