Smad4分子在ERK/MAPK信号转导通路中的作用  

Smad4 Participates in the TGF-β1-mediated ERK/MAPK Signaling Pathway

作　　者：何欣蓉[1] 霍艳英[1] 胡迎春[1] 李刚[1] 刘敏丽[2] 宋博强[3] 米粲[2] 吴德昌[1] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850 [2]重庆医科大学病理学教研室,重庆400016 [3]军事医学科学院附属医院,北京100850

出　　处：《中国生物化学与分子生物学报》2006年第4期333-337,共5页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金项目(No.30200329)~~

摘　　要：为了探讨在人永生化支气管上皮细胞BEP2D细胞中,Smad4分子对ERK/MAPK通路的作用,我们用RNA干扰的方法分别设计了两对Smad4-siRNA,并使BEP2D细胞中Smad4靶向沉默,用Western印迹分析了细胞内ERK激酶和MEK激酶磷酸化水平的变化.结果发现,当Smad4表达沉默后,ERK激酶磷酸化水平未变,MEK激酶磷酸化水平有所降低;再加TGF-β1诱导后ERK激酶和MEK激酶磷酸化水平均显著降低至基础水平以下.结果表明在BEP2D细胞中,Smad4的缺失抑制TGF-β1对ERK/MAPK通路的活化,故提出TGF-β活化ERK/MAPK通路需要Smad4存在的假设.To investigate the function of Smad4 in ERK/MAPK signaling pathway in human bronchial epithelial BEP2D cells, siRNAs were designed to specifically target Smad4 in BEP2D cells, siRNA transfection and western blots were performed to examine the phosphorylation levels of ERK and MEK. The results showed that Smad4 gene silencing leads to the phosphorylation level of MEK1 but not ERK decreased. After stimulation with TGF-β1, phosphorylation levels of both ERK and MEK are decreased and lower than the control cells. The results demonstrated that Smad4 is participating in the activation of the ERK/MAPK signaling by TGF-β1.

关 键 词：TGF-β1 SMAD4 ERK MEK RNA干涉 

分 类 号：Q257[生物学—细胞生物学] Q415