纳米晶胶原基骨与骨髓间充质干细胞复合培养对表型影响  

Effects of Nano-Hydroxyapatite/Collagen to Bone Marrow Mesenchymal Stem Cells on Phenotype Change

作　　者：孙伟[1] 李子荣[1] 张岚[2] 蔡哲[2] 李艳[3] 崔福斋[3] 

机构地区：[1]中日友好医院骨坏死与关节保留重建中心,北京100029 [2]中日友好临床医学研究所免疫室,北京100029 [3]清华大学材料科学与工程系,北京100084

出　　处：《中国误诊学杂志》2006年第8期1419-1421,共3页Chinese Journal of Misdiagnostics

基　　金：首都医学发展基金重大联合项目(编号:2002-1007);卫生部部属(管)临床学科重点项目(编号:2004-2006);中日友好医院博士启动课题(编号:20056150)

摘　　要：目的:观察纳米晶胶原基骨(nano-Hydroxyapatite/co llagen,nHAC)与骨髓间充质干细胞(M esenchym a lstem ce ll,M SC)复合培养前后的表型变化。方法:仿生原理制备nHAC,体外培养M SC并与材料复合,通过碱性磷酸酶染色和流式细胞仪观察M SC与材料的前后表型。结果:M SC可以nHAC在上贴附、生长、繁殖、分化,复合前后表型一致。结论:nHAC是良好的支架材料,易与M SC复合,适合种子细胞的贴附、生长、增殖和分化,并对细胞表型无影响,证实纳米晶胶原基骨是组织工程良好的载体材料。Objective ： To observe the effects of bone marrow mesenchymal stem cells phenotype changes before and after culturted with nano-Hydroxyapatite/Collagen （nHAC）. Methods：Prepare bone formation material, and combined with human bone marrow mesenchymal stem cells in vitro. To observe the phenotype by alkaline phosphatase staining and flow cytometry. Results ：MSC could adhere,growth ,proliferation and differentiate in nHAC. There were no change of phenotype after the combination. Conclusion：nHAC is easy to combined with MSC,and suitable for cells to adhere,grow,proliferate and differentiate, nHAC has no effects on MSC phenotype,it proves that nHAC is an excellent scaffold of tissue engineering.

关 键 词：硬羟基磷灰石 胶原 间质干细胞 组织工程 细胞 培养的 

分 类 号：R329.29[医药卫生—人体解剖和组织胚胎学]