泡沫细胞形成过程中TOLL样受体4表达的变化  

Mechanisms of increased expression of Toll-like receptor—4 during the formation of foam cells

作　　者：成蓓[1] 余其振[1] 柯丽[1] 白智峰[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院老年病科,湖北武汉市430070

出　　处：《心血管康复医学杂志》2006年第2期114-116,共3页Chinese Journal of Cardiovascular Rehabilitation Medicine

基　　金：国家自然科学基金(No.30170378)。

摘　　要：目的：研究人单核细胞系在分化为巨噬细胞及转化为泡沫细胞过程中 TOLL 样受体4(TLR-4)表达的改变及其机制。方法：体外培养人 THP-1单核细胞系,加入佛波酯(PMA)培养48h 使其分化为巨噬细胞,后者再加入氧化低密度脂蛋白(ox-LDL)使转化为泡沫细胞,采用免疫细胞化学、蛋白质印迹法和逆转录聚合酶链反应 (RT—PCR)方法,检测 TLR-4蛋白表达及 TLR-4mRNA 表达的水平。结果：在单核细胞分化为臣噬细胞的过程中 TLR-4mRNA 及蛋白水平提高,差异具有显著性(P＜0.05)；在进一步转化为泡沫细胞过程中,mRNA 及蛋白水平又增高,与巨噬细胞相比差异有显著性(P＜0.05)。结论：单核细胞分化为泡沫细胞的过程中 TLR-4转录水平上调,蛋白量合成增加,从而可能提高泡沫细胞在动脉粥样硬化中的炎性反应能力。Objective： To explore the mechanisms of increased expression of Toll-like receptor 4 （TLR-4） during the formation of foam cells. Methods ： Low density lipoprotein （LDL） was oxidized by incubation with CuCl2. The THP 1 human monocytic leukemia cell line （THP-1） was chosen in our study and cultured in RPMI 1640. The differentiation of THP-1 ceils into maerophages （MP） was induced by phorbol myristate acetate （PMA） for 48 h. The MP ceils were then incubated with oxidized LDL （ox-LDL） to generate foam ceils （FC）. The mRNA and the protein mass levels of TLR-4 were determined by immunoeytoehemistry. Western blotting and reverse transcription polyrnerase chain reaction （RT-PCR） in all three groups respectively. Results：The TLR-4 mRNA and the protein mass level were significantly increased not only during the differenciation of monocytes into macrophages （P〈0. 05 ） but also during the formation of lipid-laden foam cells （P〈0. 05 ）. Conclusion： The up regulation of TLR-4 gene expression during the differentiation of monocytes into rnacrophages and the differentiation of macrophages into foam ceils can increase TLR-4 protein synthesis dramatically, which may be closely related to ability of form scell＇s inflamma tion reaction in atherosclerosis.

关 键 词：TOLL 样受体4 巨噬细胞 动脉硬化 

分 类 号：R543.509[医药卫生—心血管疾病]