Bioeffects of Low Energy Ion-Beam Implantation and Vacuum Treatment on M_1 Arabidopsis Thaliana  

作　　者：杨根 卞坡 张正红 任杰 吴李君 余增亮 

机构地区：[1]Key Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031, China [2]Anhui University, Hefei 230031, China [3]Anhui Agricultural University, Hefei 230031, China

出　　处：《Plasma Science and Technology》2006年第2期242-246,共5页等离子体科学和技术（英文版）

基　　金：supported by National Science Fund for Distinguished Young Scholars (No. 10225526)

摘　　要：Vacuum treatment and ion-beam bombardment are two major processes in the low energy ion-beam implantation. To accurately study the contributions of these two major factors to the bioeffects separately, the M1 generation variation of Arabidopsis thaliana with ion-beam implantation and vacuum treatment were compared through a series of key plant development parameters including morphological observation, biochemical assay and RAPD （random amplified polymorphic DNA） analysis. The results showed that ion-beam implantation had obvious effect on almost all of these parameters, and the vacuum treatment had some impacts on several morphological parameters such as the bolting time and the length of the primary stem. Taking the results together, the indication is that vacuum treatment has some slight contributions to the bioeffects of ion-beam implantation while ion-beam bombardment itself is the major creator of the bioeffects.Vacuum treatment and ion-beam bombardment are two major processes in the low energy ion-beam implantation. To accurately study the contributions of these two major factors to the bioeffects separately, the M1 generation variation of Arabidopsis thaliana with ion-beam implantation and vacuum treatment were compared through a series of key plant development parameters including morphological observation, biochemical assay and RAPD （random amplified polymorphic DNA） analysis. The results showed that ion-beam implantation had obvious effect on almost all of these parameters, and the vacuum treatment had some impacts on several morphological parameters such as the bolting time and the length of the primary stem. Taking the results together, the indication is that vacuum treatment has some slight contributions to the bioeffects of ion-beam implantation while ion-beam bombardment itself is the major creator of the bioeffects.

关 键 词：BIOEFFECTS ION-BEAM VACUUM Arabidopsis thaliana 

分 类 号：O539[理学—等离子体物理]