低氧性肺动脉高压大鼠肺组织apelin-APJ系统的变化  

Changes of Apelin-APJ Signaling Pathway in Lung of Rats with Pulmonary Hypertension Induced by Hypoxia

作　　者：龚永生[1,2] 范小芳[2] 吴小脉[2] 高钰琪[1] 胡良冈[2] 毛孙忠[1] 

机构地区：[1]第三军医大学高原军事医学系病理生理学教研室,重庆400038 [2]温州医学院肺心病研究室

出　　处：《中国微循环》2006年第2期119-122,i0001,共5页Journal of Chinese Microcirculation

基　　金：温州市科委(No.Y2004A115);浙江省医药卫生(2005B126)资助项目

摘　　要：目的探讨新发现的小分子活性肽apelin及其受体APJ与缺氧性肺动脉高压病理进程的关系及意义。方法清洁级雄性SD大鼠20只,随机分为正常对照组和低氧2周组。测定平均肺动脉压(mPAP)、平均颈动脉压(mCAP)及右心室与左心室+室间隔重量比(RV/LV+S),以评价缺氧性肺动脉高压模型。放免法测定血浆、肺组织匀浆apelin水平,免疫组织化学和RT-PCR分别检测肺组织apelin、APJ蛋白及基因表达的变化。结果①缺氧大鼠mPAP较对照组高124.88%(P<0.01),RV/LV+S较对照组高25.33%(P<0.01)。两组大鼠mCAP差异无统计学意义。②肺组织及血浆apelin浓度,缺氧组与对照组比较,分别为10.58±1.02vs11.17±0.73(pg/mg.pro)和479±45vs427±63(pg/ml),P均>0.05。免疫组化显示,缺氧大鼠肺组织APJ的表达较对照组低,而apelin的表达两者差异无显著性。③缺氧大鼠肺组织apelinmRNA表达水平与对照组比较差异无统计学意义(0.925±0.058vs1.021±0.036,P>0.05),而APJmRNA的表达显著低于后者(0.944±0.106vs1.199±0.053,P<0.05)。结论APJ受体表达的异常下调所致的apelin-APJ信号系统受损,在缺氧性肺动脉高压发生、发展过程中可能扮演重要角色。Objective To investigate the probable function of apelin - APJ signaling pathway in pathological proceeding of pulmonary hypertension induced by hypoxia. Methods Twenty male Sprague-Dawley rats were randomly divided into normal control （NC） group and 2-week hypoxia （2H） group. The mPAP, reCAP and RV/LV ＋ S were measured to estimate the model of pulmonary hypertension. The concentrations of apelin in the plasma and lung tissue were measured by radioimmunity. The expression of apelin and APJ pep- tides and gene in lung tissue were measured respectively by immunohistochemistry and reverse transcriptionpolymerase chain raction （RT-PCR）. Results ①The mPAP and RV/（ LV ＋ S） of 2H group were respectively 124.88% and 25.33% higher than NC group（ P 〈 0.01）, while there was no significant difference of MCAP between the two groups. ② There was no difference of apelin concentration of lung tissue （ pg,/mg, pro） and plasma （pg/ml） between the two groups, while the expression of APJ in lung of 2H group measured by immunohistochemistry was lower than that in NC group. ③The expression of APJ mRNA in lung tissue of 2H group was lower than that in NC group（0.944±0. 106 vs 1. 199 ±0.053, P 〈0.05）, but there was no difference of apelin mRNA. Conclusion It might be an important factor in the development of pulmonary hypertension induced by hypoxia of the down-regulated expressiond of APJ gene and peptide in the lung tissue in rats.

关 键 词：APELIN 受体 缺氧 高血压 肺性 鼠 

分 类 号：R563.9[医药卫生—呼吸系统] Q514.4[医药卫生—内科学]