人脑星形细胞肿瘤微血管组织芯片的构建  

Construction of tissue microarray with astrocytic tumor microvessels of human brain

作　　者：王清良[1] 卞修武[1] 章容[1] 蒋雪峰[1] 

机构地区：[1]第三军医大学西南医院病理学研究所,重庆400038

出　　处：《第三军医大学学报》2006年第9期886-888,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目(30370552)~~

摘　　要：目的制作含有不同微血管形态的人脑星形细胞肿瘤组织芯片,并探讨其在血管生成研究中的应用价值。方法从200例人脑胶质瘤组织标本中选择含有不同类型微血管形态组织36例作为供体组织,经打孔、取样、点样等步骤,制作AstMV组织芯片,并进行CD34、FⅧ-RAg和CD105免疫组化标记,观察微血管形态特点和血管生成活性。结果成功制作出了含有不同微血管形态的组织微阵列(组织芯片)AstMV系列,自行制作了取样及点样定位器具。CD34、FⅧ-RAg及CD105均在微血管内皮细胞膜或(和)细胞质表达,不同的内皮细胞标记物所显示的微血管数有所不同,Ⅱ、Ⅲ、Ⅳ级星形细胞肿瘤中CD34、FⅧ-RAg标记的微血管数明显多于CD105标记的微血管数,亦明显多于相应Ⅰ级星形细胞肿瘤标记的微血管数。结论构建不同微血管形态的肿瘤组织芯片在血管生成研究中有重要的应用价值。本研究为进一步构建数字化肿瘤微血管以及研究星形细胞肿瘤微血管与血管生成因子间的数学模型关系奠定了基础。Objective To construct tissue microarray with astrocytic tumor microvessels （AstMV） of human brain and investigate astrocytic tumor angiogenesis. Methods Thirty-six specimens containing different microvessels from 200 specimens were selected as donor blocks, which was followed by punching, sampling and seeding samples with tissue microarray apparatus. The tissue microarrays were used to detect the expression of CD34, FVH-RAg and CD105. Results Tissue microarray of AstMV was successfully constructed and an instrument for location was made. CD34, FVH-RAg and CD105 were expressed on endothelial cell membrane or （and） plasma. There were difference in microvessel number between astrocytic tumors and between endothelial markers. In the grade Ⅱ, Ⅲ and Ⅳ astrocytomas, microvessels labeled by CD34 and FVH-RAg antibodies were more than those detected by CD105 antibody. There were number difference in microvessels labeled by CD34 and FVH-RAg antibodies between grade Ⅰ and grade Ⅱ, Ⅲ and Ⅳ tumors. Conclusion Construction of tissue microarray containing different tumor microvessels might be of significance in evaluating tumor angiogenesis. This study may be the basis for constructing digital tumor microvessel models and exploring the mathematic relation between astrocytic tumor microvessels and vascular growth factors.

关 键 词：星形细胞肿瘤 组织芯片 血管生成 

分 类 号：R318.04[医药卫生—生物医学工程] R322.13[医药卫生—基础医学]