外源性高胰岛素血症对血管内皮细胞sICAM-1表达的影响  

Effect of exogenous hyperinsulinism on soluble ICAM-1 expression of rabbit vascular endothelial cells

作　　者：刘兆川[1] 王旭开[1] 杨立[1] 杨成明[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所心血管内科,重庆400042

出　　处：《第三军医大学学报》2006年第9期936-938,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目(30170384)~~

摘　　要：目的通过观察高胰岛素血症对血管内皮细胞可溶性细胞间黏附分子-1(sICAM-1)表达的影响,探讨其在动脉粥样硬化(atherosc lerosis,AS)形成和发展中的作用。方法新西兰白兔40只,随机分为4组:正常对照组、高胰岛素血症组、高糖高胆固醇组、联合组。每日皮下注射胰岛素,建立外源性高胰岛素血症。通过放射免疫法测血清胰岛素水平,酶联免疫吸附实验(ELISA法)测定血清sICAM-1水平,免疫组化法测血管内皮细胞sICAM-1的表达。结果与对照组相比,高胰岛素血症组、高糖高胆固醇组、联合组3组血清sICAM-1水平均明显升高(P<0.05),联合组显著高于高胰岛素血症组和高糖高胆固醇组;高胰岛素血症组和高糖高胆固醇组sICAM-1表达水平相似,但低于联合组。结论胰岛素可增强血管内皮细胞sICAM-1表达,且与高糖高胆固醇饮食有协同增强作用。Objective To explore soluble ICAM-1 （ slCAM-1 ） expression in the vascular endothelial cells and investigate the role of hyperinsulinism in the formation and the progress of artherosclerosis. Methods Forty New Zealand albino rabbits were randomly divided into four group （ n = 10 each group）： control group in which the rabbits were fed with special forage for rabbits and everyday were subcutaneously injected of physiological saline for 8 weeks, hyperinsulinism group in which the rabbits were of special forage and subcutaneous injection of 6 U/kg insuline, high glucose and cholesterol （HGC） group in which the rabbits were of HGC forage and subcutaneous injection of saline, hyperinsulinism ＋ HGC group in which rabbits were of HGC forage and subcutanous injection of hyperinsulinism. The serum level of slCAM-1 was measured by ELISA before the experiment, 2, 4, 6, 8 weeks after the experiment beginning. The expression of sICAM-1 in the vascular endotheial cells was observed by histochemical method in 8 weeks. Results As compared with control group, the serum level of sICAM-1 in hyperinsulinism group, HGC group, hyperinsulinism ＋ HGC group increased significantly with the highest level in hyperinsulinism ＋ HGC group. The expression of sICAM-1 in the vascular endotheial cells was higher in the three groups than control group, and the highest expression were observed in hyperinsulinism ＋ HGC group. Conclusion Insuline may augment the expression of sICAM-1 in the vascular endotheial cells, promote the progress of artherosclerosis.

关 键 词：高胰岛素血症 黏附分子 可溶性细胞问黏附分子-1 动脉粥样硬化 

分 类 号：R322.12[医药卫生—人体解剖和组织胚胎学] R446.11[医药卫生—基础医学]