口蹄疫病毒R株基因组序列分析研究  

Genome Sequence Analysis of Foot-and-Mouth Disease Virus R Strain

作　　者：林小敏[1] 孙彦伟 余业东 罗满林[1] 林绍荣[1] 贺东生[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]广东省动物防疫监督总所,广东广州510230

出　　处：《华南农业大学学报》2006年第2期118-120,共3页Journal of South China Agricultural University

基　　金：广东省科技计划项目(2003A20403);广东省农业厅动植物科研项目粤农[2004]314号

摘　　要：根据GenBank上发表的口蹄疫（foot-and-mouth disease，FMD）全基因序列数据，设计了多对引物，采用RT-PCR的方法，分别对R株编码区和非编码区基因进行扩增，将各片段克隆至pMD18-T载体，进行核苷酸序列测定．按顺序拼接各基因的序列，将结果序列与GenBank上各FMDV毒株的序列进行比较和同源性分析．序列的比较和分析表明，R株编码区全长为6966个核苷酸，共编码2322个氨基酸；非编码区5’端内部核糖体结合位点（intemal ribosome entry site，IRES）长约450个核苷酸，3’非翻译区（untranslatable region，UTR）从TAA始至Poly（A）前长度为94个核苷酸，所测出Poly（A）尾长度为18个核苷酸．与GenBank世界流行毒株的序列比较，编码区核苷酸序列同源性为88％～90％，推导氨基酸序列同源性为88％～95％．Several pairs of primers were designed to amphfy the complete genome of foot-and-mouth disease virus R strain by RT-PCR. Each fragment was cloned to pMD18-T vector and sequenced. Analysis showed that the length of the encoding region of R strain was 6 966 bp, encoding for 2 322 amino acid. The 5＇IRES region and 3＇UTR were approximately 450 bp and 94 bp in length. Eighteen nucleotide acid was detected in Poly （A） region by 3＇ RACE. Compared with other FMDV strains＇ sequences in GenBank, the encoding region of R strain had 88% ～ 90% similarities in nucleotide sequence and 88% ～ 95% similarities in deduced amino acid sequence.

关 键 词：口蹄疫病毒 基因组 序列分析 

分 类 号：S852.659.6[农业科学—基础兽医学]