中国南方部分城市泌尿生殖道沙眼衣原体基因分型研究  

Genotyping of Chlamydia trachomatis isolated from patients with sexually transmitted diseases from four cities in Southern China

作　　者：陈丽丽[1] 吴移谋[1] 邓仲良[1] 蔡恒玲[1] 余敏君[1] 顾伟民[2] 张俊芳[3] 

机构地区：[1]南华大学病原生物学研究所,湖南衡阳421001 [2]上海市皮肤病性病医院 [3]南华大学第二附属医院

出　　处：《中华皮肤科杂志》2006年第5期275-277,共3页Chinese Journal of Dermatology

基　　金：湖南省科技厅重点项目基金(04SK2003)

摘　　要：目的 了解中国南方4个城市性病高危人群中沙眼衣原体（Ct）基因型的分布特征。方法 从衡阳、上海、广州、江门四个城市的性病门诊收集1180份泌尿生殖道标本，经质粒PCR筛选后，阳性者用巢式PCR扩增Ctompl基因片段，酶切omp1扩增产物，分析Ct各临床株omp1基因的限制性片段长度多态性（RFLP），将临床株分型。结果 Ct质粒PCR阳性者301份，用于分型者286份。通过RFLP分型，共检出10个基因型，其中常见的是E（30．8％）、J（23．8％）、D（17．5％）、F（10．5％）、G（6．2％）型，并且不同城市间基因型的分布差异无统计学意义（r=0．981，P〉0．05）。结论 4个城市存在Ct多种基因型，并且基因型的分布大致相同。Objective To investigate the distribution of Chlaraydia trachomatis genotypes in high risk populations of STDs from four cities in Southern China. Methods C trachomatis was detected by a plasmid PCR in 1180 specimens collected in Hengyang, Shanghai, Guangzhou and Jiangmen, and then the major outer membrane protein gene （ompl） was amplified by nested PCR （nPCR） with plas- mid-PCR-positive specimens. The amplicons of omp I were digested by restriction endonuclease Alu I, Msp I, EcoR I, or Dde I. C. trachomatis was genotyped according to the restriction fragment length polymorphism （ RFLP ） patterns. Results Of 301 plasmid-PCR-positive specimens, 286 （48 collected from Hengyang, 32 from Shanghai, 52 from Guangzhou and 154 from Jiangmen） could be genotyped. The most prevalent genotype was serotype E（ 30.8% ）, followed by J（ 23.8% ）, D（ 17.5% ）, F（ 10.5% ）, and G （ 6.2% ）. The distribution of various genotypes was not different among different cities. Conclusion Polymorphic genotypes of C .trachomatis are prevalent in Hengyang, Shanghai, Guangzhou and Jiangmen, and there is no significant difference of genotype distribution among these four cities.

关 键 词：衣原体 沙眼 基因 omp1 多态性 限制性片段长度 

分 类 号：R759[医药卫生—皮肤病学与性病学]