肝包虫周围纤维囊壁中骨桥蛋白免疫印记与组化分析  

Analysis of osteopontin in pericystic layer of hepatic hydatid cyst by Western blotting and immunohistochemical technique

作　　者：李建辉[1] 彭心宇[1] 唐娟[2] 吴向未[1] 张示杰[1] 牛建华[1] 孙红[1] 牛少雄[1] 吕游[1] 

机构地区：[1]石河子大学医学院第一附属医院普外一科 [2]新疆石河子大学医学院组胚教研室

出　　处：《世界华人消化杂志》2006年第7期693-696,共4页World Chinese Journal of Digestology

基　　金：国家自然科学基金攻关项目;No.30260108教育部科学技术研究重点项目;No.03162

摘　　要：目的:研究肝细粒棘球蚴(肝包虫)周围纤维囊壁中骨桥蛋白(osteopontin,OPN)的表达定位．方法:采用免疫印记法检测OPN在肝细粒棘球蚴(n=48)周围纤维囊壁中的表达,并结合免疫组化方法观察其组织学分布．结果:肝细粒棘球蚴周围纤维囊壁经免疫印记分析80％有OPN表达,形态学观察集中分布于近虫体侧纤维性囊壁(内层),与近肝侧纤维性囊壁(外层)比较有显著差异(75％ vs 8．3％, P<0．05)．结论:OPN参与肝细粒棘球蚴周围内层纤维性囊壁肉芽肿反应的调节．AIM： To investigate the expression and distribution of osteopontin （OPN） in hepatic hydatid cyst and its significances. METHODS： Proteins extracted from 60 surgically excised hydatid cyst tissues were subjected to polyacrylamide gel electrophoresis followed by Western blotting, using polyclonal antibodies directed against OPN; the distribution of OPN was also observed by immunohistochemical techniques. RESULTS： OPN expression was detected in 48（80%） cases of cysts by Western blotting. OPN was mainly distributed in the side of pericystic layer near the parasite, which was significantly higher than that distributed near the liver （75% vs 8.3%, P 〈 0.05）. CONCLUSION： OPN plays a regulated role in the granuloma reaction of the pericystic layer of hepatic hydatid cyst.

关 键 词：细粒棘球蚴 骨桥蛋白 免疫印记 肝包虫 周围纤维囊壁 

分 类 号：R532.32[医药卫生—内科学] R575[医药卫生—临床医学]