实时荧光定量PCR检测糖尿病大鼠视网膜中转化生长因子β受体-1和受体-2基因表达(英文)  

Expression of transforming growth factor-β type 1 receptor and transforming growth factor type 2 receptor in diabetic rat retina

作　　者：曹丹[1] 柳林[1] 沈炜[1] 

机构地区：[1]第二军医大学附属长海医院眼科,中国上海市200433

出　　处：《国际眼科杂志》2006年第2期247-251,共5页International Eye Science

基　　金：中国国家自然科学基金资助项目(No.30271391);中国上海市卫生局科研项目(No.034124);中国上海市卫生系统百名跨世纪优秀学科带头人培养计划(百人计划)基金资助项目(No.057)~~

摘　　要：目的:定量检测转化生长因子β受体-1(TransformingGrowthFactor-βreceptortype1,TβR1)和转化生长因子β受体-2(TransformingGrowthFactor-βreceptortype2,TβR2)编码基因在糖尿病大鼠视网膜中不同时相点的动态表达水平,以探讨转化生长因子β及转化生长因子β受体在糖尿病视网膜病变(DR)中的作用。方法:选择健康成年SD大鼠28只,随机分为正常对照组(CON)和糖尿病组(DM)。利用链尿佐菌素(STZ)诱导大鼠糖尿病视网膜病变模型,制备RNA并逆转录,实时荧光定量PCR技术分析TβR1和TβR2在不同时相点相对于内参照基因18s的相对mRNA含量。结果:TβR1在4,12wk时相对于18s的mRNA含量分别为0.000493±0.000133和0.000608±0.000232。TβR2在4,12wk时相对于18s的mRNA含量分别为0.000166±0.000057和0.000113±0.000049。TβR1基因表达水平随着病变进展呈上升趋势,TβR2在4wk时表达量较正常增加,到12wk时表达量又减少,逐渐恢复到正常水平。结论:糖尿病大鼠视网膜中TβR1和TβR2基因表达水平变化可能影响TGF-β信号的转导过程,TGF-β及其受体介导的信号转导通路在糖尿病视网膜病变中具有重要作用。AIM： To quantitatively detect gene expression level of transforming growth factor-β type 1 receptor （TβR1） and transforming growth factor-β type 2 receptor （TβR2） in different stage of diabetic rats＇ retina. To observe and analyze the effect of transforming growth factor-β receptors on the retina of rat diabetic animal model. METHODS： 28 healthy adult Sprague-Dawley rats were chosen and randomly divided into two groups of normal control （CON） and diabetes mellitus （DM）. Diabetes was induced by streptozotocin （STZ） intraperitoneal injection. Gene expression was detected quantitatively with real-time fluorescence quantitative reverse transcription polymerase chain reaction （QRT-PCR）. RESULTS： The mRNA level of TβR1 and TβR2 was 0.000493±0.000133 and 0.000166±0.000057 at 4wk. The mRNA level of TβR1 and TβR2 was 0.000608±0.000232 and 0.000113±0.000049 at 12wk. TβR1 expression was gradually elevated during the progression of diabetic retinopathy, TβR2 expression was up-regulated at 4wk, but down-regulated at 12wk. CONCLUSION： TGF-β and its receptors （TβR1 and TβR2） may play important role in the pathogenesis of diabetic retinopathy.

关 键 词：糖尿病视网膜病变 转化生长因子-β 受体 链尿佐菌素 实时荧光定量PCR 基因表达 

分 类 号：R774.1[医药卫生—眼科]