RhBMP-2诱导犬气管移植体软骨再生的实验研究  

Recombinant human bone morphogenetic protein-2 stimulation of cartilage regeneration in canine tracheal graft

作　　者：李小飞[1] 汪健[1] 张涛[1] 卢强[1] 程庆书[1] 刘锟[1] 

机构地区：[1]第四军医大学唐都医院胸外科,西安710038

出　　处：《中华胸心血管外科杂志》2006年第2期118-122,共5页Chinese Journal of Thoracic and Cardiovascular Surgery

基　　金：国家自然科学基金(39970707);陕西省自然科学基金(99SM37)资助

摘　　要：目的探讨重组人骨形态发生蛋白-2(rhBMP-2)植入犬气管移植体内诱导软骨再生效果。方法12只犬随机等分两组,分别将rhBMP-2/胶原(collagen)或胶原植入犬气管移植段,进行自体移植,通过HE染色、Masson’s染色、免疫组化检测软骨再生。18只犬随机等分为6组,给予不同浓度的rhBMP-2,利用HPIAS-1000高清晰度彩色病理图像分析仪测定各组新生软骨的面积,确定最佳诱导浓度。12只犬随机等分成2组,分别进行自体、异体移植并植入rhBMP-2,测定各组移植段气管管腔及新生软骨面积。结果rhBMP-2/胶原植入气管移植段后,可以在植入区及固有软骨的软骨膜处诱导软骨再生,新生软骨面积与对照组差异有统计学意义(P<0.01)。rhBMP-2剂量与新生软骨面积呈剂量依赖关系,当rhBMP-2浓度为5mg/ml时,其诱导的新生软骨面积最多。在自体移植与异体移植实验中,植入rhBMP-2后,移植段气管管腔的狭窄情况均较对照组得到改善。结论rhBMP-2可促进气管移植体软骨再生,其诱导作用不仅可以促进植入区的软骨再生,而且通过软骨细胞间的接触促进了软骨膜处的软骨再生。可见rhBMP-2可以促进移植段气管的软骨再生,克服气管移植段的软化狭窄。Objective To induce regeneration of dog＇s tracheal cartilage by implanting the recombinant human bone morphogenetic protein-2 （rhBMP-2） into the tracheal graft in order to prevent the trachea from collapse. Methods RhBMP-2 with the ateloptide type-1 collagen cartier was implanted around the cartilage ring of the autograft. New cartilage formed was detected with the histological analysis and immunohistochemical stain after 4 weeks. Six different concentrations of rhBMP-2 with collagen carrier were implanted round the cartilage ring in 6 auto transplantation groups respectively. The area of new cartilage regeneration was calculated in choosing the optimal dosage of rhBMP-2. Auto and allo tracheal transplantation were performed with rhBMP-2 implanted arond the cartilage ring, the diameter of the grafts, histdogical analysis, and the area of the new cartilage regeneration were calculated. Results The addition of rhBMP-2 resulted in significantly greater amount of the new cartilage area （ P 〈 0.01）. The area of new cartilage increased with increasing the concentration of rhBMP-2. A concentration of 5 mg/ml was likely the most effective dosage. In both the auto and allo grafts, new cartilage was found within the tracheal cartilage rings and around perichondrium. There was significant increase in the diameter of the trachea indicating was cicatrical stenosis following implanting rhBMP-2. Conclusion This study dentonstinted that rhBMP-2 may significantly stimulate the cartilage regeneration in the peri-implant sites and aroud the periehondrium. Conclusively it is suggested that rhBMP-2 can be used to stimulate cartilage growth of tracheal grafts in onter to prevent the stenosis after tracheal transplantation.

关 键 词：气管 生物假体 软骨 再生 

分 类 号：R653[医药卫生—外科学] R655.3[医药卫生—临床医学]