HSF1抑制热应激所致RAW264.7巨噬细胞凋亡  

HSF1 inhibits heat stress-induced apoptosis in Raw264.7 macrophages

作　　者：鄂顺梅[1] 肖卫民[1] 王慷慨[1] 王秋鹏[1] 刘梅冬[1] 刘可[1] 肖献忠[1] 

机构地区：[1]中南大学湘雅医学院病理生理教研室,长沙410078

出　　处：《中南大学学报（医学版）》2006年第2期162-166,共5页Journal of Central South University ：Medical Science

基　　金：国家973重点项目(G2000056908);国家自然科学基金(30470851;30270533);教育部博士点基金(20020533032)

摘　　要：目的:探讨热休克因子1(heat shock factor 1,HSF1)对热应激所致Raw2 6 4.7巨噬细胞凋亡的影响。方法:采用热应激(4 2.5℃±0.5℃)处理稳定表达小鼠HSF1基因的Raw2 6 4.7巨噬细胞1h,3 7℃分别恢复6,9,1 2,2 4 h,采用流式细胞术,hoechst3 3 2 5 8染色和DNA琼脂糖凝胶电泳检测细胞凋亡。结果:流式细胞术结果显示,热应激后对照组(转空载体)细胞凋亡核百分率较热应激前明显升高,9 h达峰值(约为6 0%),此时荧光染色可见3 0%的细胞出现核固缩,凋亡小体等典型的凋亡形态学改变;并于热应激后6,9,1 2 h均能检测到清晰的DNA梯状条带。与转空载体对照组相比,HSF1过表达能显著降低热应激所致凋亡及明显抑制DNA的断裂。结论:HSF1可以抑制热应激所致的Raw2 6 4.7巨噬细胞凋亡。Objective To observe the effect of heat shock factor 1 （ HSF1 ） on heat stress-induced apoptosis in Raw264.7 macrophages. Methods Raw264.7 cells transfected with pcDNA3. 1 and pcDNA3. 1-HSF1 were exposed to heat stress （42.5 ℃ ±0.5 ℃ ） for 1 h and recovered at 37 ℃ for 6, 9, 12, and 24 h respectively. Flow cytometry （FCM） , Hoechst 33258 staining and DNA ladder assays were performed to assess the apoptosis. Results After heat stress, FCM showed that apoptotic cells were increased significantly and reached the peak at 9 h in Raw 264. 7 cells transfected with pcDNA3. 1 , and were characterized with classical morphologic changes including apoptotic body and nuclear condensation. Agarose gel electrophoresis showed that ＂ DNA ladder＂ could be observed clearly at 6, 9, and 12 h after the heat stress. But the overexpression of HSF1 could reduce the number of apoptotic cells and inhibit DNA fragmentation. Conclusion HSF1 can inhibit heat stress-induced apoptosis in Raw264.7 macrophages.

关 键 词：热休克因子1 凋亡 热应激 RAW264.7巨噬细胞 

分 类 号：R363[医药卫生—病理学]