造血刺激因子对巨核细胞体外扩增的影响  

Effect of hematopoietic stimulating factors on the expansion of megakaryocyte

作　　者：黄艳红[1] 王绮如[1] 

机构地区：[1]中南大学湘雅医学院血液生理研究室,长沙410078

出　　处：《中南大学学报（医学版）》2006年第2期204-207,共4页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(39970092)

摘　　要：目的:探讨造血刺激因子对巨核系成熟细胞体外扩增的影响。方法:将小鼠骨髓单个核细胞(BMNC)按(2,4,6,8,1 0)×1 05个/mL浓度加入半固体培养体系,体外培养巨核细胞集落形成单位(CFU-M eg),观察BMNC数与CFU-M eg生成数之间的关系;将BMNC分别加入含rmSCF+rmTPO+rm IL-3(3HSFs),rmSCF+rm IL-3+rmTPO+rm IL-6(4HSFs)或骨髓成纤维细胞条件培养液(F-CM)的液体培养体系中持续培养,从培养的第1 d开始每两天观察巨核系成熟细胞的生成情况。结果:当小鼠骨髓单个核细胞按(2,4,6,8,1 0)×1 05个/mL浓度种入CFU-M eg培养体系时,CFU-M eg生成数随细胞种入数增加而增多;种入细胞浓度对CFU-M eg产率有影响,种入细胞浓度为1×1 06个/mL时,与种入细胞浓度为2×1 05个/mL,每2×1 05个骨髓单个核细胞生成的CFU-M eg数相比,差异有统计学意义(P<0.0 5)。3HSFs,4HSFs和F-CM均对巨核系成熟细胞体外扩增有促进作用。3HSFs和4HSFs的扩增作用明显优于F-CM,4HSFs的扩增效果优于3HSFs;3HSFs和4HSFs扩增体系扩增7 d效果最好,F-CM扩增体系扩增5 d效果最好。结论:3HSFs,4HSFs和F-CM均对巨核系成熟细胞体外扩增有促进作用;4HSFs扩增成熟巨核细胞效果优于3HSFs和F-CM;不同扩增体系扩增巨核细胞的最佳效果所需的时间不同。Objective To investigate the effect of hematopoietic stimulating factors on the expansion of mature megakaryocytes. Methods （ 2,4,6,8,10 ）×10^5 / mL bone marrow single nucleus cells （BMNC） were added in the culture system of colony forming unit-megkaryocyte （CFU- Meg ） to find out the relationship of the cultured BMNC with the output of CFU- Meg . rmSCF ＋ rmT- PO ＋ rmIL-3 （3HSFs） and rmSCF ＋ rmTPO ＋ rmIL-3 ＋ rmIL-6 （4HSFs） or F-CM were added in the liquid culture system of megkaryocytes respectively. The number of mature megakaryocytes were counted every other day. Results The number of CFU-Meg increased with the increase of the cultured BMNC. The CFU-Meg productivity of 1 × 10^6 BMNC/mL culture system was more than that of 2 × 10^5 BMNC/mL culture system. 3 HSFs and 4HSFs or F-CM significantly promoted the expansion of mature megakaryocytes in the liquid culture system, but the effect was different. The peak time of the number of mature megakaryocytes in 3HSFs and 4HSFs or F-CM were 7 d, 7 d and 5 d respectively. Conclusion 3 HSFs and 4 HSFs or F-CM had positive effect on the expansion of mature megakaryocytes . 4HSFs was better than 3HSFs and F-CM. 3HSFs was better than F-CM. The peak time of the number of mature megakaryocytes in different culture systems was different.

关 键 词：巨核系集落形成单位 巨核细胞 造血 刺激因子 

分 类 号：R33[医药卫生—人体生理学]