类风湿性关节炎患者Th1/Th2、Tc1/Tc2细胞的检测及意义  

Detection of the Th1/Th2、Tc1/Tc2 Subsets in Rheumatoid Arthritis Patients and Its Significance

作　　者：欧国生 

机构地区：[1]新兴县人民医院,广东新兴527400

出　　处：《实用医技杂志》2006年第6期849-851,共3页Journal of Practical Medical Techniques

摘　　要：目的:探讨类风湿性关节炎(rheumatoidarthritis,RA)患者外周血Th1/Th2、Tc1/Tc2细胞亚群极化状态,分析RA的免疫学发病机制,为RA的临床治疗提供理论依据。方法:收集RA患者及健康者外周抗凝静脉血,分离纯化淋巴细胞。FITC标记的抗CD3单抗,Cy5标记的抗CD4、CD8单抗,PE标记的抗CD30单抗,以CD3/CD4、CD3/CD8设门作三色流式细胞术(Tris-colorflowcytometry),分析RA患者T细胞亚群极化状态。结果:与正常对照相比,RA患者外周血CD4+细胞百分率显著升高(P<0.01),CD8+细胞百分率则无明显变化(P>0.05),CD4+/CD8+比例明显升高(P<0.05);CD3+CD4+CD30-细胞百分率、CD4+CD30-/CD4+CD30+和CD8+CD30-/CD8+CD30+比例均明显升高(P<0.01),而CD3+CD4+CD30+、CD3+CD8+CD30-及CD3+CD8+CD30+细胞百分率无明显变化(P>0.05)。结论:RA患者外周血存在细胞免疫功能失调,T细胞亚群极化状态发生改变,呈明显的Th1类细胞优势;Th1/Tc1型细胞介导的细胞免疫可能与RA的发病机制有关。Objective To investigate the polarization of T cells subset and analyze the immunologic pathogenesis in RA. Methods The ratios of CD4^＋/CD8^＋ ,CD3^＋ CD4^＋ CD30^-/CD3^＋ CD4^＋ CD30^＋ and CD3^＋ CD8^＋ CD30^- /CD3^＋ CD8^＋ CD30^＋ in peripheral blood T cells were analyzed by immunofluorescence staining and tris-color flow cytometry in vitro. Results Compared with the ratios of CD4^＋/CD8^＋ （1.57±0.04） in healthy people,the ratios of CD4^＋/CD8^＋ （1. 95 ±0.05） in RA in creased obviously （P〈0.05）. Compared with the ratios of CD3^＋CD4^＋CD30^-/CD3^＋ CD4^＋ CD30^＋ （ 15.74 ± 5. 36 ） and CD3^＋ CD8^＋ CD30^-/CD3^＋ CD8^＋ CD30^＋ （ 15.82 ± 4.03 ） inhealthy people, theratiosof CD3^＋ CD4^＋ CD30^-/CD3^＋ CD4^＋ CD30^＋ （20.72±7.34） and CD3^＋ CD8^＋ CD30^-/CD3^＋ CD8^＋ CD30^＋ （ 19.81 ± 3.28）. FCM analysis showed that average percentages of CD4^＋ （47.42%±2.68%） in RA increased sharply （P〈0.01） versus 40.27%±2. 53% in healthy people, while the average percentages of CD8^＋, CD3^＋ CD4^＋ CD30^＋, CD3^＋ CD8^＋ CD30^- and CD3^＋ CD8^＋ CD30^＋ did not changed versus healthy people. Conclusion The ratios of CD4^＋/CD8^＋ ,CD3^＋ CD4^＋ CD30^-/CD3^＋ CD4^＋ CD30^＋ and CD3^＋ CD8^＋ CD30^-/CD3^＋ CD8^＋ CD30^＋ in peripheral blood T cells were increased obviously in RA. It suggested that cellular immunity in RA is shifted to Thl type immunity.

关 键 词：类风湿性关节炎 T细胞亚群 三色流式细胞术 

分 类 号：R593.22[医药卫生—内科学]