卵泡刺激素受体表达与卵巢肿瘤发病的关系  

Relationship of mRNA and Protein of Follicle-stimulating Hormone Receptor and Ovarian Tumor

作　　者：杜涌瑞[1] 高燕[1] 卢奕[2] 李会强[2] 高企贤[1] 姚智[2] 

机构地区：[1]天津市中心妇产科医院,300052 [2]天津医科大学免疫学教研室

出　　处：《天津医药》2006年第4期241-243,共3页Tianjin Medical Journal

基　　金：天津市卫生局资助项目(项目编号:01KY48)

摘　　要：目的:探讨卵泡刺激素受体与上皮性卵巢肿瘤的关系。方法:应用半定量逆转录-聚合酶链式反应(RT-PCR),免疫印迹(Westernblot)检测10例对照组,30例良性组及32例恶性组的卵泡刺激素受体(FSHR)mRNA和蛋白的表达情况。结果:FSHRmRNA在对照组中均表达(10/10),良性组中表达率为96.7%(29/30),恶性组中表达率为59.4%(19/32);其蛋白在对照组中均表达(10/10),良性组中表达率为90%(27/30),恶性组中表达率为46.9%(15/32),恶性组中FSHRmRNA及蛋白表达率明显低于对照组和良性组(均P<0.01),而且FSHR的表达程度,从对照组到良性组再到恶性组呈下降趋势。结论:在卵巢癌组织中卵泡刺激素受体的表达率及表达程度显著降低,卵巢癌细胞的增殖并非完全依赖促性腺激素-卵泡刺激素。Objective： To investigate the relationship of follicle-stimulating hormone receptor （FSHR） and epithelial ovarian cancer. Methods： Ten normal ovaries, thirty benign ovarian tumors, thirty-two epithelial ovarian carcinomas were examined for FSHR mRNA and protein expression by semi-quatitive reverse transcription polymerase reaction （RT-PCR） and Western blot. Results： Expression of FSHR mRNA was found in 100%（10/10） of normal ovaries, 97.6% （29/30） of benign ovarian tumors, 59.4 % （19/32） of epithelial ovarian carcinoma. On the other hand, the protein expression in three tissues was 100% （10/10）, 90% （27/30） and 46.9% （15/32）respectively .The expression rate of FSHR mRNA and protein in ovarian carcinoma tissue was significantly lower than that in the normal ovarian tissue and benign ovarian tumors （P 〈 0.01）. What is more,the expression degree of FSHR had a decrease tendency in order from malignant, normal ovarian tissue to the epithelial ovarian carcinoma. Conclusion： The expression rate and degree of FSHR in the cpithelial ovarian cancer tissue significantly decrease,the proliferation of ovarian cancer cells is not thoroughly dependent on gonadotropic hormone-follicle stimulating hormone.

关 键 词：卵巢肿瘤 卵泡刺激素 逆转录聚合酶链反应 

分 类 号：R979.21[医药卫生—药品] R737.31[医药卫生—药学]