小麦耐盐相关基因LCT多样性研究  

Study on Diversity of Wheat Salt-Tolerance LCT Gene

作　　者：李孟军[1] 周荣华[1] 贾继增[1] 

机构地区：[1]中国农业科学院作物科学研究所农业部作物种质资源与生物技术重点开放实验室,北京100081

出　　处：《华北农学报》2006年第2期6-9,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家重点基础研究发展973计划(2004CB117200)

摘　　要：LCT1可能是普通小麦Na+和Cs+吸收的一条重要途径。因此,发掘小麦品种中LCT1自然变异,对于通过常规育种或基因工程提高小麦耐盐性具有重要意义。根据小麦离子转运蛋白LCT1(low-affinity cation transporter)基因 cDNA序列(NCBI登录号AF015523),设计引物对8个小麦品种中LCT多样性进行了初步研究。通过克隆测序和全序列比对在8个小麦品种中发现10种LCT序列,分别命名为LCT2-1,LCT2-2,LCT2-3,LCT3-1,LCT3-2,LCT3-3,LCT3- 4,LCT3-5,LCT3-6和LCT3-7。结果表明:10种LCT均与LCT1存在差异。LCT2-1,LCT2-3,LCT3-2,LCT3-3,LCT3-4, LCT3-6和LCT3-7序列发生碱基缺失,缺失均发生在其氨基端。10种LCT序列单碱基突变既有颠换又有转换且均为有义突变。不同小麦品种含有的LCT种类差别较大,其中茶淀红最多(4种),而百农3217和苏麦3号仅发现1种。在 6个小麦品种中都发现LCT3-1。LCT1 is probably one of important way of absorbing Na^＋ and Cs^＋ in bread wheat （ Triticum aestivum L. ）. Thereafter, Mining LCT1 natural diversity in wheat is important to improve salt-tolerance by traditional breeding and genetics engineering. PCR primers corresponding to LCT1 cDNA sequence（NCBI Accession： AF015523） was used to the study on LCT natural diversity in eight wheat accessions. Based on LCT sequences blast, 10 kinds of LCT sequences were found. They were named LCT2-1, LCT 2-2, LCT2-3 , LCT3-1, LCT3-2 , LCT3-3 , LCT3-4 , LCT3-5 , LCT3- 6 and LCT3-7. The result showed that 10 kinds of LCT sequences are all different from LCT1. Deletion mutations in LCT2-1, LCT2-3 , LCT3-2 , LCT3-3 , LCT3-4 , LCT3-6 and LCT3-7 were all happened on LCT N-terminal . Point mutants in 10 kinds of LCT sequences all led to amino-acid change. The LCT kinds were different in different wheat accessions. Four LCT kinds were found in Chadianhong and only one in Bainong 3217 and Sumai 3. LCT3-1 exists in six wheat accessions.

关 键 词：小麦 耐盐性 LCT 多样性 

分 类 号：S512.01[农业科学—作物学]