丛枝菌根对喜树幼苗喜树碱含量的影响  

Effect of arbuscular mycorrhiza on camptothecin content in Camptotheca acuminata seedlings

作　　者：赵昕[1] 王博文[1] 阎秀峰[1] 

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040

出　　处：《生态学报》2006年第4期1057-1062,共6页Acta Ecologica Sinica

基　　金：黑龙江省杰出青年基金资助项目(JC0211)~~

摘　　要：喜树(Camptotheca acuminata)是我国特有的多年生亚热带落叶阔叶树种,因其次生代谢产物喜树碱具有良好的抗肿瘤活性而受到人们的广泛关注。通过温室盆栽接种试验,观察了2属6种丛枝菌根真菌对喜树幼苗喜树碱含量的影响。结果表明,接种的6种丛枝菌根真菌与喜树幼苗均形成了共生体系并且发育良好。透光球囊霉(Glomusdiaphanum)、幼套球囊霉(G.etunicatum)、蜜色无梗囊霉(Acaulosporamellea)和光壁无梗囊霉(A.laevis)侵染形成丛枝菌根有利于提高喜树幼苗的喜树碱含量,地表球囊霉(G.versiforme)则影响不大,而木薯球囊霉(G.manihot)却降低了喜树幼苗的喜树碱含量。丛枝菌根形成对喜树幼苗喜树碱代谢的影响还表现在喜树碱的器官分配上,菌根幼苗根中的喜树碱比例均高于无菌根幼苗。Mycorrhizal fungi form the most important mutualistic symbioses on earth with plants. Much research has shown that the development of arbuscular mycorrhize （AM） is correlated with plant secondary metabolism. AM fungi can directly or indirectly affect plant secondary metabolic processes. Camptothecin （CPT）, a secondary metabolite in a special Chinese tree Camptotheca acuminata, has gained great attention for its remarkable inhibitory activity against tumor cells. The effect of AM infected with six fungi belonging to two genera on CPT content in C. acuminata seedlings was carried out in the present study. The selected sterile seeds of C. acuminata were sown in sterilized sands in the greenhouse. After 70 days of cultivation, seedlings with similar height and crown were selected and divided into seven groups （10 pots per group）. Each group of C. acuminata seedlings was inoculated with one of AM fungus Glomus manihot, G. versiforme, G. etunicatum, G. diaphanum, Acaulospora mellea, A. laevis, or non-mycorrhizal inoculation, namely Gin, Gv, Ge, Gd, Am, A1, or control （CK）, respectively. After 3-month cultivation, the symbiotic association was observed and CPT content in the seedlings of C. acuminata was determined. All C. acuminata seedlings inoculated with six fungi formed AM. Colonization rates of AM was over 70% in seedlings infected with 4 Glomus fungi （Gin, Gv, Ge, Gd）, and ～ 50% with 2 Acaulospora fungi. Mycorrhizal colonization intensity of roots （M） and infected root fragments （m） were showed in Table 1. Good symbiosis systems were formed between C. acuminata seedlings and selected mycorrhizal fungi. In roots, CPT contents in mycorrhizal seedlings were significantly higher than non-mycorrhizal seedlings, except Gin. CPT contents in Gd, Am, Al, Ge and Gv were 2.5, 2.1, 1.7, 1.6, and 1.3 times higher as CK, respectively. In the stem, CPT content was significantly higher in Al than CK, no significant difference among CK, Ge, Gd, and Am, while significantly lower in Gm and Gv

关 键 词：喜树幼苗 丛枝菌根 喜树碱 次生代谢 

分 类 号：S567.19[农业科学—中草药栽培]