离子胁迫诱导洋葱鳞茎内表皮细胞凋亡  

Apoptosis Induced by Ionic Stress in Endocuticle Cells of Onion Squamous Bulb

作　　者：李昊文[1] 李鹏[1] 印莉萍[1] 

机构地区：[1]首都师范大学生命科学学院,北京100037

出　　处：《生物技术通报》2006年第1期69-72,共4页Biotechnology Bulletin

基　　金：首都师范大学精品课建设基金资助

摘　　要：通过不同浓度离子胁迫诱导剂(NaCl、CaCl2)对洋葱鳞茎内表皮细胞进行不同时间的处理,发现0.1M、0.5M的NaCl和CaCl2处理2小时即可诱导出细胞凋亡现象,随处理时间延长直至10小时,细胞核凋亡的形态学变化和凋亡小体更加明显,基因组DNA降解更加梯状条带化。本实验对离子诱导的洋葱鳞茎内表皮细胞凋亡现象做了较系统的描述,为植物细胞凋亡的研究及细胞凋亡实验教学提供了经济、快捷、有效的诱导方法。Ionic stress （NaCl, CaCl2 ） caused apoptosis in endocuticle cells of onion squamous bulb only within two hours by different concentrations （0.1M and 0.5M respectively）. With extending treatment in NaCl, CaCl2 （2 to 10 hours）, the cell nuclei were highly vacuolated and DNA were greatly fragmented in endocuticle cells. Meanwhile, the apoptosis body and DNA ladder could be clearly observed like animal cells. This paper made detailed description about plants＇ apoptosis inducted by ionic stress. We could like to provide an economical, speedy and effective method for the apoptosis research and experimental teaching.

关 键 词：细胞凋亡 离子胁迫 洋葱 

分 类 号：Q942[生物学—植物学]