左旋卡尼汀预处理对大鼠移植心脏缺血再灌注时心肌细胞凋亡的影响  

Effect of L-carnitine on apoptosis following rat heart transplantation

作　　者：田立群[1] 郅兴义[1] 

机构地区：[1]郑州大学第一附属医院心脏外科,郑州市450052

出　　处：《实用诊断与治疗杂志》2006年第5期346-347,共2页Journal of Practical Diagnosis and Therapy

摘　　要：目的:探讨左旋卡尼汀(L-CN)预处理对移植心脏再灌注时细胞凋亡的影响。方法:采用大鼠心脏移植模型,随机分为3组:正常组(A组)、对照组(B组)、和实验组(C组)。术后3 h测定各组心肌TNFα的含量及心肌含水量,检测心肌细胞凋亡指数。结果:与A组相比,B组和C组心肌TNFα、心肌含水量和心肌细胞凋亡指数均明显升高(P<0.05)。与B组相比,C组心肌TNFα、心肌含水量及心肌细胞凋亡指数均明显降低(P<0.05)。结论:左旋卡尼汀预处理能有效抑制心肌TNFα的表达,减轻心肌水肿,抑制心肌细胞凋亡,从而减轻心肌再灌注损伤。Objective To investigate the effects of L-carnitine （L-CN） pretreatment on apoptosis during the stage of ischemia-reperfusion. Methods The rat model of heterotopic heart transplantation was set up. Wistar rats were randomly divided into three groups： normal group （A）, control group （B） and experimental group （C）. Three hours after operation, the levels of TNF a and the percentage of myocardial water of cardiac allograft were detected, and apoptosis was also examined. Results The levels of TNFα, the percentage of myocardial water and apoptosis index in group B and C were significantly higher than in group A （P〈0.05）, and significantly lower in group C than in group B. Conclusion L-carnitine pretreatment can decrease the expression of TNF α,improve myoedema and inhibit apoptosis,so as to prevent reperfusion injury in rat cardiac transplant models.

关 键 词：心脏移植 细胞凋亡 肿瘤坏死因子Α 左旋卡尼汀 

分 类 号：R96[医药卫生—药理学]