流动力对细胞原癌基因c-fos和c-myc表达的影响  

The Effects of the Fluid Forces on the Expression of the Proto-Oncogenes c-fos and c-myc

作　　者：丁库克[1] 许莉莉[1] 王春燕[1] 黄菊英[1] 胡金麟[2] 宋晓燕[3] 曾衍钧[3] 

机构地区：[1]首都医科大学生物医学工程学院,北京100069 [2]中国人民解放军总医院微循环研究室,北京100853 [3]北京工业大学生物医学工程中心,北京100022

出　　处：《北京生物医学工程》2006年第2期189-192,共4页Beijing Biomedical Engineering

基　　金：国家自然科学基金(39600040;10172096);北京市自然基金(3982005)资助

摘　　要：目的研究流体力对脑微血管内皮细胞原癌基因cf-os和c-myc表达的影响,为探索微血管疾病机理提供一定实验依据。方法获取Wistar大鼠脑微血管进行细胞培养,在平行板流动小室中对所培养的细胞施加力学作用,并应用免役组织化学技术观察微血管内皮细胞原癌基因cf-os和c-myc的表达。结果不同的流动力对体外培养的大鼠脑微血管内皮细胞cf-os和c-myc蛋白表达产生了不同的影响。cf-os蛋白表达在静态时非常低,但在4dynes/cm2和10dynes/cm2的流体力的作用下可诱导cf-os蛋白水平迅速增加,尤其是10dynes/cm2的流体力,并随着时间的延长而增加,到1h时达到高峰,随后cf-os蛋白表达量出现下降,2.5h时接近基础水平。c-myc蛋白在静态时表达也非常低,经流体力作用后缓慢增多。结论流体力的大小会影响cf-os和c-myc的表达,血液的力学信号传入细胞内并引起细胞的一些应答,也印证了cf-os和c-myc是被血液流体力作用短暂上调的基因,流体力与内皮细胞生长、增殖、损伤也有直接关系。Objective To investigate the effects of fluid forces on the expression of proto-oncogenes c-fos and c-myc in order to get some experimental data for exploring the mechanism of diseases relating to microvessel. Method Cell was cultured after taking out the brain microvessl of Wister rats, and put in the fluid chamber with moving liquid. Then the measurement of gene expression about c-fos and c-myc by technology of immunohistochemistry was performed. Result The effects of several fluid forces on the expression of proto-oncogenes c-fos and c-myc in cultured rat brain endothelial cells are different.The c-los protein levels are very low in the stationary state. The flttid forces of 4 dynes/cm^2 and 10 dynes/cm^2 rapid increases the c-fos protein levels, especially when the shear stress is 10 dynes/cm^2. The levels raise along with the time going and peak at 1 .0h, and then begin to decrease. They decline to the basal level in 2.5h. The c-myc protein levels are also very low in the static state. They increase after the onset of shear stress slowly. Conclusion The shear forces of fluid affected the expression of c-fos and c-myn. The cells of brain microvessel were respensed to the signal stimulation from fluid force. This study proves that c-fos and c-myn are two quickly - rising genes, which are dirctly related to cell growth, reproduction and damnification.

关 键 词：流体力 原癌基因 C-FOS C-MYC 

分 类 号：R318.01[医药卫生—生物医学工程]