信号核酸识体用于药物托普霉素的高灵敏度检测  

High Sensitive Tobramycin Detection Using a Novel Signaling Aptamer

作　　者：周翠松[1] 江雅新[2] 汪俊[2] 麻宝成[2] 李梦龙[1] 方晓红[2] 

机构地区：[1]四川大学化学学院,成都610064 [2]中国科学院化学研究所,北京100080

出　　处：《高等学校化学学报》2006年第5期826-829,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20225516;90406006)资助

摘　　要：提出了一种利用RNA核酸识体和光开关化合物[Ru(phen)2dppz]2+(RU)高灵敏检测药物分子的荧光分析新方法.以托普霉素(TOB)为靶分子,检测限可达到10 nmol/L,同时该方法有较好的选择性,能在溶液中实现无需分离的实时检测.这种无标记检测方法有利于不稳定RNA核酸识体在分析检测中的应用,简化实验操作,为其它药物分子的检测提供了新思路.Aptamers are a new class of oligonucleotides generated from in vitro selection with a high affinity and specificity. In this paper, a novel luminescence detection method was developed by using RNA aptamers and a DNA light switching complex, [ Ru （phen）2 （dppz） ] ^2＋, as a signaling aptamer for the detection of drug tobramycin. The results showed that the method was highly sensitive and selective with the detection limit down to 10 nmol/L. The method was also very simple and could realize direct detection without separation in solution, and offers a new approach for the exploitation and application of aptamers in drug analysis.

关 键 词：核酸识体 托普霉素 荧光光谱法 光开关化合物 核糖核酸 

分 类 号：O657.31[理学—分析化学]