毛细管电泳直接分析与水不互溶溶液中痕量物质的新方法  

A Novel Method for Capillary Electrophoresis Directly Analyzing Trace Amounts Analytes in the Water-immiscible Solution Samples

作　　者：方怀防[1] 曾昭睿[1] 刘岚[1] 庞代文[1] 

机构地区：[1]武汉大学化学与分子科学学院,武汉430072

出　　处：《高等学校化学学报》2006年第5期856-858,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20375028);国家"八六三"计划项目(批准号:2002AA2Z2004)资助

摘　　要：This paper describes a novel method in which solvent microextraction（SME） is followed by on-line back-extraction-field amplified sample injection（OLBE-FASI） in capillary zone electrophoresis（CZE）.The water-immiscible solution sample, sealed with water plug in the sample vial,was used for direct electroinjection. The water plug with moderate content of organic solvent,low-conductivity,and the presence of a small（amount） of H+ provided the highest sensitivity for analyzing positively chargeable compounds,such as cocaine and thebaine.The linear range was 0.040—4,0.040—2 μg/mL,with the square of the correlation coefficient(r2)>0.997,respectively.Detection limits were in the range of 5—10 ng/mL.This paper describes a novel method in which solvent microextraction （SME） is followed by on-line back-extraction-field amplified sample injection （OLBE-FASI） in capillary zone electrophoresis （CZE）. The water-immiscible solution sample, sealed with water plug in the sample vial, was used for direct electroinjection. The water plug with moderate content of organic solvent, low-conductivity, and the presence of a small amount of H ＋ provided the highest sensitivity for analyzing positively chargeable compounds, such as cocaine and thebaine. The linear range was 0. 040---4, 0. 040--2 μg/mL, with the square of the correlation coeffi- cient（r^2） 〉0. 997, respectively. Detection limits were in the range of 5--10 ng/mL.

关 键 词：溶剂微萃取 在线反萃取 场放大进样 

分 类 号：O657[理学—分析化学]