大脑中动脉栓塞模型大鼠的中枢电压依赖性钾通道mRNA表达的改变  

mRNA expression of voltage-dependent potassium channels in the brain of rats after middle cerebral artery occlusion

作　　者：张海霞[1] 李正斌[1] 王晓良[1] 

机构地区：[1]中国医学科学院中国协和医科大学药物研究所,北京100050

出　　处：《药学学报》2006年第4期328-332,共5页Acta Pharmaceutica Sinica

基　　金：国家重点基础研究发展计划(973计划)资助项目(2004CB518906);国家自然科学基金资助项目(30271490;30371644).

摘　　要：目的观察几种代表性电压依赖性钾通道亚型在脑缺血不同时间大鼠海马和皮层mRNA表达水平的变化。方法采用大脑中动脉栓塞模型致大鼠脑缺血损伤,应用RT-PCR方法检测Kv1.4,Kv1.5,Kv2.1和Kv4.2mRNA表达水平在海马和皮层中的改变。结果大脑中动脉栓塞模型大鼠出现明显的神经损伤症状。缺血2 h时,海马组织的Kv1.4,Kv2.1和Kv4.2 mRNA表达水平分别增加了50%,67%和90%,在缺血24 h时Kv1.4和Kv4.2mRNA仍保持高水平表达。大鼠皮层组织在缺血2 h后,Kv1.4,Kv1.5,Kv2.1和Kv4.2 mRNA水平均无明显改变,缺血24 h后,Kv2.1和Kv4.2 mRNA水平分别增加了70%和62%。结论大脑中动脉栓塞模型大鼠的海马和皮层组织中电压依赖性钾通道亚型的mRNA表达发生明显上调。Aim To study the mRNA expression changes in the brain of rats after middle cerebral artery occlusion. Methods Middle cerebral artery occlusion was used to induce ischemia in rat brain. The mRNA expression of voltage-dependent potassium channel subtypes, including Kv1. 4, Kv1. 5, Kv2. 1 and Kv4.2, were detected in rat hippocampus and cortex by RT-PCR. Results Middle cerebral artery occlusion induced a significant neurological injury in rats. After ischemia 2 h, the mRNA of Kv1. 4, Kv2. 1 and Kv4.2 in hippocampus increased by 50% , 67% and 90% , respectively. And the mRNA of Kvl. 4 and Kv4.2 maintained at a high level in hippocampus after ischemia 24 h. In cortex, the mRNA level of all the four subtypes were not changed significantly after ischemia 2 h, but the mRNA of Kv2. 1 and Kv4.2 increased by 70% and 62% after ischemia 24 h, respectively. Conclusion The mRNA expression levels of voltage-dependent potassium channels were up-regulated in rat hippocampus and cortex after middle cerebral artery occlusion.

关 键 词：钾通道 逆转录聚合酶链式反应 大脑中动脉栓塞 MRNA表达 

分 类 号：R965.2[医药卫生—药理学]