反相离子对色谱法测定附子中生物碱成分  

Determination of alkaloids in Radix Aconiti Lateralis Preparata by RP-ion-pair HPLC

作　　者：刘秀秀[1] 晁若冰[1] 

机构地区：[1]四川大学华西药学院,四川成都610041

出　　处：《药学学报》2006年第4期365-369,共5页Acta Pharmaceutica Sinica

摘　　要：目的测定附子中乌头碱、新乌头碱、次乌头碱、北乌碱、苯甲酰乌头原碱和苯甲酰新乌头原碱等6种生物碱的含量。方法用反相离子对HPLC法,使用A ichromBond-1 C18柱(250 mm×4.6 mm ID);流动相:乙腈-5 mmol.L-1NaH2PO4溶液,磷酸调至pH 4.5(50∶50),内含7 mmol.L-1十二烷基硫酸钠(SDS);检测波长:235 nm;流速:1.0 mL.m in-1;柱温:35℃。结果以上6种生物碱可以完全分离,准确测定。结论该方法准确度高,可应用于附子中生物碱的含量测定。Aim To separate and quantitatively determine six alkaloids： aconitine, mesaconitine, hypaconitine, beiwutine, benzoylaconine and benzoylmesaconine in the Chinese traditional medicine Radix Aconiti Lateralis Preparata （Fuzi）. Methods A RP-ion-pair HPLC method was established. An AichromBond-1 C18 column was used at a column-temperature of 35 ℃. The mobile phase was CH3CN- 5 mmol·L^-1 NaH2PO4（50：50） containing 7 mmol·L^-1 SDS at a flow-rate of 1.0 mL · min^-1. The detector was set at UV 235 nm. Results These six alkaloids can be completely separated and determined quantitatively. Conclusion This method is accurate and suitable for the determination of six alkaloids in Fuzi.

关 键 词：附子 高效液相色谱法 乌头碱 新乌头碱 次乌头碱 北乌碱 苯甲酰乌头原碱 苯甲酰新乌头原碱 

分 类 号：R917[医药卫生—药物分析学]